Reviews on methods of analysis

The recent developments in the determination of acetylcholine and choline, the advantages and the disadvantages of a variety of analytical methods used in the analysis, were reviewed and discussed by Maruyama et al. [7]. Hanin published an overview for the methods used for the analysis and measurement of acetylcholine [8], Shimada et al. presented a review, including the applications of some reactors in the analysis of choline and acetylcholine. The immobilized enzyme reactors were used for detection systems in high performance liquid chromatography [9], 

Tsai reviewed the separation methods used in the determination of choline and acetylcholine [10]. This review surveyed the array of analytical techniques that have been adopted for the measurement of acetylcholine or its main precursor/metabolite (choline), ranging from simple (bioassay, radio enzymatic assay, gas chromatography-flame ionization detection, gas chromatography-mass spectrometry, high 

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