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Reversed-phase chromatography glycoproteins

A. General description Eptifibatide is a cyclic heptapeptide containing six amino acids and one mercaptopropionyl residue. An interchain disulfide bridge is formed between the cysteine amide and the mercaptopropionyl moieties. Eptifibatide binds to the platelet receptor glycoprotein (gp) Ilb/IIIa of human platelets and inhibits platelet aggregation. The eptifibatide peptide is produced by solution-phase peptide synthesis, and is purified by preparative reverse-phase liquid chromatography and lyophifized. [Pg.154]

DEH Palladino, RM House, KA Cohen. Measurement of amino acid compositions of glycoprotein systems by gas-phase hydrolysis and reversed-phase high-performance chromatography. J Chro-matogr 599 3-11, 1992. [Pg.88]

Hancock, W. S. Apffel, A. Chakel, J. Souders, C. M Timkulu, T. Pungor, E. Guzzetta, A. W. 1996. Reversed-phase peptide mapping of glycoproteins using liquid chromatography/electrospray ionization-mass spectrometry. Methods Enzymol., 271,4QA-421. [Pg.215]

Medzihradszky, K. F. Besman, M. J. Burlingame, A. L. 1998. Reverse-phase capillary high performance liquid chromatography/high performance electrospray ionization mass spectrometry an essential tool for the characterization of complex glycoprotein digests. Rapid Commun. Mass Spectrom., 12,472-478. [Pg.223]

Kwon H and Kim J (1993) Determination of monosaccharides in glycoproteins by reverse-phase high-performance liquid chromatography. Analytical Biochemistry 215 243-252. [Pg.432]

Kurihara T, Min JZ, Toyo oka T, Fukushima T, Inagaki S. Determination of fluorescence-labeled asparaginyl-oUgosaccharide in glycoprotein by reversed-phase ultraperformance liquid chromatography and time-of-flight mass spectrometry. Anal Chem 2007 79 8694-8. [Pg.54]

The on-line combination of LC-LC-MS has been investigated for a number of reasons. In addition to the general prospects of multidimensional separation techniques, especially enhanced selectivity, there was special interest in the ability to perform LC-LC with two different mobile-phase compositions. In this way, it should be possible to avoid problems with mobile-phase incompatibility due to the use of nonvolatile mobile-phase constituents. A good example of this approach is the determination of enantiomers of p-blockers in plasma samples, described by Edholm and co-workers. Racemic mixtures of a p-blocker like metoprolol can be separated on a ai-acid glycoprotein column. However, the chromatography requires the use of a 20 mM phosphate buffer (pH 7) in the mobile phase, which is not compatible with on-line LC-MS. Therefore, the chiral column was coupled via a set of two trapping columns to a common reversed-phase LC column. After separation, the two enantiomers were sepa-... [Pg.846]

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See also in sourсe #XX -- [ Pg.641 , Pg.642 , Pg.643 ]



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Chromatography reverse

Glycoprotein phases

Phases chromatography

Reverse-Phased Chromatography

Reverse-phase chromatography

Reversed-phase chromatography

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