Reversed-phase chromatography biomedical applications

This review describes currently useful methods for the separation and characterization of inorganic complexes. Some separations are required just to remove unreacted reagents or minor side products. Advances for these standard techniques (ion chromatography, normal and reversed-phase chromatography, thin-layer methods) are often limited by the availability of a specialized support material. However, newer, innovative methods for the separation of species have evolved, particularly separation methods needed in biomedical and industrial applications. 

A.M. Krstulovic and P.R. Brown, Reversed-Phase High Performance Liquid Chromatography Theory, Practice and Biomedical Applications, Wiley, New York, 1982. 

H7. Hearn, M. T. W., and Hancock, W. S, The role of ion-pair reversed phase HPLC in peptide and protein chemistry. In Biological/Biomedical Applications of Liquid Chromatography II (G. L. Hawk, ed.), pp. 243-271, Dekker, New York, 1979. 

Water ean usually be used as a unique solvent for the study of biomedical materials. In other applications such as reverse-phase liquid chromatography, however, different solvents such as water-methanol mixed solvents are used. As the MeOH fraction increases, the surface tension of the solution decreases. In mixed solvents, the trend of contact angles as a function of concentration was generally similar to that of the aqueous case. As the proportion of methanol increases, the contact angle deereases dramatically as in the case of decreased concentration. 

Krstulovic A M, Brown P R 1982 Reversed phase high-performance liquid chromatography theory, practice, and biomedical applications. John Wiley New York, 296 pp... 

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