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Reverse transcriptase-PCR

Rokbi, B., Seguin, D., Guy, B., Mazarin, V., Vidor, E., Mion, F., Cadoz, M., and Quentin-Millet, M.-J. (2001). Assessment of Helicobacter pylori gene expression within mouse and human gastric mucosae by real-time reverse transcriptase PCR. Infect. Immun. 69, 4759 766. [Pg.156]

Genetic methods Polymerase chain reaction (PCR) and its variants, e.g., multiplex PCR, are widely used for C. botulinum detection. Reverse-transcriptase PCR is recommended as an alternative to biological tests using mice (McGrath et al., 2000). [Pg.205]

Furthermore, passage of LCM across the blood-brain barrier would be facilitated by the fact that scavenger receptors (both type I and II, of class A) have been shown, by a reverse transcriptase PCR study of messenger RNA expression, to be present normally in (bovine) brain microvessels (ref. 678). Brain microvessels therefore appear to play an active role in the uptake of native and modified LDL (ref. 678) accordingly, these micro vessels would be expected to have a similar role in LCM uptake (cf. Sections 12.1 and 14.2.2 above see also below). [Pg.250]

Downstream application [PCR (polymerase chain reaction), cloning, labeling, blotting, RT (reverse transcriptase)-PCR, cDNA synthesis, RNAse protection assays, gene therapy, etc.]... [Pg.333]

Lanciotti RS, Kerst AJ, Nasci RS, et al. Rapid detection of West Nile virus from human clinical specimens, field-collected mosquitoes, and avian samples by a TaqMan reverse transcriptase PCR assay. J Clin Microbiol. 2000 38 4066-4071. [Pg.80]

N, Northern blotting RT, reverse transcriptase PCR ISH, in situ hybridization. [Pg.179]

It is mentioned also that the presence of the enzyme revCTse transcriptase may serve to furnish a marker. (This enzyme is otherwise known as RNA-directed DNA polymerase, and is the enzyme distinguishing retroviruses.) The conversion of isolated RNA to cDNA, is involved followed by amplification, a process designated as reverse transcriptase-PCR, or simply RT-PCR. [Pg.182]

In 2002, an up-to-date report by Xu et al. [25] shows that an extraction of fibrinolytic enzymes from L. bimastus contains three proteins with apparent molecular masses about 30,000, 29,000 and 28,000 Da in SDS-PAGE, respectively. According to the N-terminal sequences, a cDNA is obtained by reverse transcriptase PCR (RT-PCR). The cDNA is inserted in a vector and confirmed by sequencing. The results indicate that the fragment encodes a 242-amino-acid protein called PV242 [26]. The whole amino ficid sequence is 35% homologous to either F-1I1-1 or -HI-2. [Pg.827]

We searched for isoprenyl diphosphate synthase gene sequences in P. abies using a homology-based approach. First, RNA was isolated from bark and wood of methyl jasmonate-treated spruce saplings from a single clone. This was then used as a template for reverse transcriptase PCR carried out with degenerate primers... [Pg.10]

Fig. 5 Reverse transcriptase-PCR analysis of NnAChR messenger RNA (mRNA) in rat vagal pulmonary sensory neurons. Cytoplasm of 20 isolated pulmonary sensory neurons was collected into a single PCR tube. One-step Reverse transcriptase-PCR and a nested PCR were carried out to detect the presence of nicotinic mRNA subunits. The nested PCR products were run on a 1% agarose gel and stained with ethidium bromide. M 100-bp DNA marker. (From Gu et al. 2008, with permission)... Fig. 5 Reverse transcriptase-PCR analysis of NnAChR messenger RNA (mRNA) in rat vagal pulmonary sensory neurons. Cytoplasm of 20 isolated pulmonary sensory neurons was collected into a single PCR tube. One-step Reverse transcriptase-PCR and a nested PCR were carried out to detect the presence of nicotinic mRNA subunits. The nested PCR products were run on a 1% agarose gel and stained with ethidium bromide. M 100-bp DNA marker. (From Gu et al. 2008, with permission)...
Nowak B, MuefflingTV, KuefenA, GanseforthK, SeyboldtC (2005). Detection of bovine central nervous system tissue in liver sausages using a reverse transcriptase PCR technique and a commercial enzyme-linked immunosorbent assay. J. Food Prot., 68 2178-2183. [Pg.207]

Schmidt, P., Thiele, M., Rudroff, C., Vaz, A., Schilli, M., Friedrich, K., etal.. Detection of tumor cells in peritoneal lavages from patients with gastrointestinal cancer by multiplex reverse transcriptase PCR. Hepatogastroenterology 48, 1675-1679 (2001). [Pg.109]


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PCR

RT-PCR (reverse transcriptase

RT-PCR (reverse transcriptase-polymerase chain

Reverse transcriptase-polymerase chain reaction RT-PCR)

Transcriptase

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