Single-photon fluorescence resonance energy transfer

Although much of the book focuses on physical theory, 1 have emphasized aspects of optical spectroscopy that are especially pertinent to molecular biophysics, and 1 have drawn most of the examples from this area. The book therefore covers topics that receive little attention in most general books on molecular spectroscopy, including exciton interactions, resonance energy transfer, single molecule spectroscopy, high-resolution fluorescence microscopy, femtosecond pump-probe spectroscopy, and photon echoes. It says less than is customary about atomic spectroscopy and about rotational and vibrational spectroscopy of... 

Keywords FCS - GFP DsRed Cross-correlation analysis Single molecule In vivo Two-photon excitation FRET Blinking - Fluorescence resonance energy transfer... 

Keywords Fluorescence lifetime imaging microscopy Frequency domain Time-correlated single photon counting Fluorescence resonance energy transfer Protein-protein interactions... 

Chlorophyll molecules absorb light as individual photons. Each can cause a single photochemical reaction. If there is no direct photochemical reaction, chlorophyll may lose its excitation energy as heat and red-fluorescence, or by resonance transfer. In fluorescence a high energy (short wavelength) photon is absorbed, which promotes an electron. The electron then drops to the lowest... 

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