Resonance energy transfer principles

More recently double stranded DNA-binding dyes, (e.g., SYBR Green), have been introduced (Giulietti et al. 2001) which removed the need for an expensive, specific probe to be designed. Other sophisticated tools have been developed to work in conjunction with the Taqman method, for example molecular beacons, scorpions and hybridisation probes. These techniques rely on the FRET (Fluorescence Resonance Energy Transfer) principle but do not require the nuclease activity of the Taq polymerase. The different real-time... 

More recently, the method of scanning near-field optical microscopy (SNOM) has been applied to LB films of phospholipids and has revealed submicron-domain structures [55-59]. The method involves scanning a fiber-optic tip over a surface in much the same way an AFM tip is scanned over a surface. In principle, other optical experiments could be combined with the SNOM, snch as resonance energy transfer, time-resolved flnorescence, and surface plasmon resonance. It is likely that spectroscopic investigation of snbmicron domains in LB films nsing these principles will be pnrsned extensively. 

Forster, Th 211, 278, 282, 285 Forster resonance energy transfer, 282 Forster singlet energy transfer, 378 Franck-Condon factors, 23 Franck-Condon principle, 5 Franck-Condon transition, 5 French, C. S., 555 Friedman, G., 353 Fritzsche, J., 37 Frosch, R. P 252, 267, 269 Fumaronitrile, photodimerization in solid state, 478... 

Szollosi, J., Damjanovich, S., Nagy, P., Vereb, G. and Matyus, L. (2006). Principles of resonance energy transfer. Curr. Prot. Cell Biol. 1.12.11-11.12.16. 

Cheung H. C. (1991) Resonance Energy Transfer, in Larowicz J. R. (Ed.), Topics in Fluorescence Spectroscopy, Vol. 2, Principles, Plenum Press, New York, pp. 127-176. 

Principle of operation of a fluorescence resonance energy transfer biosensor. 

Rc. The fluorescence emission of a solution containing two fluorophores varies with excitation wavelength, which is not the case in the presence of one fluorophore. d The principle of resonance energy transfer. 

Figure 16.25 Semiconductor nanoparticle-based fluorescent sensors (a) Forster resonant energy transfer (FRET) between two nanoparticles induced by analyte, (b) crown ether receptor for potassium ions, and (c) operation principle of maltose fluorescent sensor. (Adapted from Chen et at. [144] and Medintz et at. [146])...

Details on the mechanisms and theories of excitation energy transfer via dipole-dipole interaction (FRET Forster resonance energy transfer) and via exchange interaction (Dexter s mechanism) can be found in B. Valeur, Molecular Fluorescence. Principles and Applications, Wiley-VCH, Weinheim, 2002, chap. 4 and 9. 

Coskun A, Akkaya EU. Signal ratio amplification via modulation of resonance energy transfer proof of principle in an emission ratiometric Hg(II) sensor. J Am Chem Soc 2006 128 14474-5. 

Figure 4.16 Schematic to show the principles of fluorescence resonance energy transfer (FRET). Top...

Current methods combine at least one of four different principles of allelic discrimination (hybridization, primer extension, ligation, or restriction) with one of four different detection techniques (chemiluminescence/ fluorescence, fluorescence polarization, resonance energy transfer, and mass spectrometry). Assay formats range from (slab)- gel electrophoresis, plates, particles, fibre arrays, and microchip arrays to semi- and homogenous assays that do not require any further sample separation or purification. 

Cardiillo, R. A. (2007) Theoretical principles and practical considerations for fluorescence resonance energy transfer microscopy. Methods Cell Biol. i, A79-A9A. 

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