High performance liquid chromatography resolution

In order to achieve better chromatographic resolution, high performance liquid chromatography was employed to determine which of the multinitropyrenes may be present in the particle extract. The TLC separation was still used as a preparative technique to isolate a multinitropyrene fraction from the... 

Coupled High Performance Liquid Chromatography with High Resolution Gas Chromatography... 

Y. Oda, N. Asakawa, Y. Yoshida and T. Sato, On-line determination and resolution of the enantiomers of ketoprofen in plasma using coupled achiral-cliiral high-performance liquid chromatography , 7. Pharm. Biomed. Anal. 10 81-87 (1992). 

High performance liquid chromatography (HPLC) has been by far the most important method for separating chlorophylls. Open column chromatography and thin layer chromatography are still used for clean-up procedures to isolate and separate carotenoids and other lipids from chlorophylls and for preparative applications, but both are losing importance for analytical purposes due to their low resolution and have been replaced by more effective techniques like solid phase, supercritical fluid extraction and counter current chromatography. The whole analysis should be as brief as possible, since each additional step is a potential source of epimers and allomers. 

B.G.M. Vandeginste, W.Derks andG. Kateman, Multicomponent self modelling curve resolution in high performance liquid chromatography by iterative target transformation analysis. Anal. Chim. Acta, 173 (1985) 253-264. 

McLaughlin, L. W. and Bischoff, R., Resolution of RNA using high-performance liquid chromatography, /. Chromatogr, 418, 51, 1987. 

Siezen, R. J., Kaplan, E. D., and Anello, R. D., Superior resolution of y-crystal-lins from microdissected eye lens by cation-exchange high-performance liquid chromatography, Biochem. Biophys. Res. Comm., 127, 153, 1985. 

Hydride Generation Atomic Absorption Spectrometry High Performance Liquid Chromatography High Performance Thin-Layer Chromatography High Resolution... 

Mal tsev, V.G., Nasledov, D.G., Trushin, T.B., Tennikova, L.V., Vinogradova, I.N., Volokitina, I.N., Zgonnik, V.N. (1990). High-performance liquid chromatography of proteins on short capillary columns. J. High Resolut. Chromatogr. 13, 185-192. 

Ferretti, R., Gallinella, B., La, T.F., Zanitti, L. (1988). Direct resolution of a new antifungal agent, voriconazole(UK-109,496) and its potential impurities, by use of coupled achiral-chiral high-performance liquid chromatography. Chromatographia 47, 649-654. 

Oda, Y., Asakawa, N., Kajima, T., Yoshida, Y., Sato, T. (1991). Column-switching high-performance liquid chromatography for online simultaneous determination and resolution of enantiomers of verapamil and its metabolites. Pharm. Res. 8, 997-1001. 

Acidimetric, spectrophotometric, and high-performance liquid chromatography (HPLC) assays were developed for the determination of 2,3,5,6,7,8-hexahydro-l//-pyrido[l,2-c]pyrimidine-l,3-diones 100 <1998APH193>. Its solubility properties were also characterized. Resolution of the enantiomers of 4-phenyl-2- 4-[4-(2-pyrimidinyl)piperazinyl]-butyl perhydropyrido[l,2-r-]pyrimidine-l,3-dione was achieved on heptakis(2-A V-dimethylcarbamoyl)-P-... 

Aboul-Enein and Ali [78] compared the chiral resolution of miconazole and two other azole compounds by high performance liquid chromatography using normal-phase amylose chiral stationary phases. The resolution of the enantiomers of ( )-econazole, ( )-miconazole, and (i)-sulconazole was achieved on different normal-phase chiral amylose columns, Chiralpak AD, AS, and AR. The mobile phase used was hexane-isopropanol-diethylamine (400 99 1). The flow rates of the mobile phase used were 0.50 and 1 mL/min. The separation factor (a) values for the resolved enantiomers of econazole, miconazole, and sulconazole in the chiral phases were in the range 1.63-1.04 the resolution factors Rs values varied from 5.68 to 0.32. 

Okamoto et al [85] performed the optical resolution of primaquine and other racemic drugs by high performance liquid chromatography using cellulose and amylose tris-(phenylcarbamate) derivatives as chiral stationary phases. Primaquine and other compounds were effectively resolved by cellulose and/or amylose derivatives having substituents such as methyl, tertiary butyl, or halogen, on the phenyl groups. 

Thick-layer silica gel chromatography can also be employed [7], although most separations are now accomplished by high-performance liquid chromatography. Resolution of complex mixtures often requires both normal and reverse phase modes [19]. A further dimension is added, when bioactivity is correlated with spectroscopically-monitored chromatographic profiles. 

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