Resolution interactions

Brick, R, Bhat, T.N., Blow, D.M. Structure of tyrosyl-tRNA synthetase refined at 2.3 A resolution. Interaction of the enzyme with the tyrosyl adenylate intermediate. /. Mol. Biol. 208 83-98, 1988. 

The comparison between measured data and simulated data are good for both real and imaginary parts. The measured signal has a low resolution due to the low interaction between the eddy current and the slot. 

The weld depths penetration for gold-nickel alloy and tantalum cylinders have been well controlled by an entirely contactless ultrasound method. Nevertheless, the development of signal and image processing will allow to increase the resolution of the ultrasonic images. Moreover, in order to be able to size quite well the lacks of weld penetration, the simulation of the interaction beam-defect is presently developed in our laboratory. 

The molecular constants that describe the stnicture of a molecule can be measured using many optical teclmiques described in section A3.5.1 as long as the resolution is sufficient to separate the rovibrational states [110. 111 and 112]. Absorption spectroscopy is difficult with ions in the gas phase, hence many ion species have been first studied by matrix isolation methods [113], in which the IR spectrum is observed for ions trapped witliin a frozen noble gas on a liquid-helium cooled surface. The measured frequencies may be shifted as much as 1 % from gas phase values because of the weak interaction witli the matrix. 

Thus, by measuring the intemiolecular vibrations of a WBC, ultimately with resolution of the rotational, tuimelling and hyperfme stmcture, the most sensitive measure of the IPS is accessed directly. The difficulty of measuring these VRT spectra is the fact that they lie nearly exclusively at THz frequencies. As expected, the stiffer die interaction, the higher in frequency these modes are found. In general, the total 0.3-30 THz interval must be accessed, although for the softest or heaviest species the modes rarely lie above 10-15 THz. 

The principal dilTerence from liquid-state NMR is that the interactions which are averaged by molecular motion on the NMR timescale in liquids lead, because of their anisotropic nature, to much wider lines in solids. Extra infonnation is, in principle, available but is often masked by the lower resolution. Thus, many of the teclmiques developed for liquid-state NMR are not currently feasible in the solid state. Furthemiore, the increased linewidth and the methods used to achieve high resolution put more demands on the spectrometer. Nevertheless, the field of solid-state NMR is advancing rapidly, with a steady stream of new experiments forthcoming. 

This angular dependence is different from the first-order perturbations so that the conventional teclmique of removing linebroadening in solids, MAS (see below), caimot completely remove this interaction at the same time as removing the first-order broadening. Flence, the resolution of MAS spectra from quadnipolar nuclei is usually worse than for spin-2 nuclei and often characteristic lineshapes are observed. If this is the case, it is... 

Ach antages. DOR works well if the quadnipolar interaction is dominant and the sample is highly crystalline, with some extremely impressive gains in resolution. Provided that the conect RF-excitation conditions are employed the spectral infonuation is directly quantitative. 

The resolution eidiancenient stems from the fact that the qiiadnipole frequencies for both transitions are correlated. At specific times the anisotropic parts of the qiiadnipole interaction are refocused and an echo fonns. The frequency of an (m, -m) transition is given by... 

Even for a single radical tire spectral resolution can be enlianced for disordered solid samples if the inliomogeneous linewidth is dominated by iimesolved hyperfme interactions. Whereas the hyperfme line broadening is not field dependent, tire anisotropic g-matrix contribution scales linearly with the external field. Thus, if the magnetic field is large enough, i.e. when the condition... 

While the spatial resolution in classical microscopy is limited to approximately X/2, where X is the optical wavelength (tlie so-called Abbe Limit [194], -0.2 pm with visible light), SNOM breaks through this barrier by monitoring the evanescent waves (of high spatial frequency) which arise following interaction with an... 

This corresponds to the physician s stethoscope case mentioned above, and has been realized [208] by bringing one leg of a resonatmg 33 kHz quartz tiinmg fork close to the surface of a sample, which is being rastered in the x-y plane. As the fork-leg nears the sample, the fork s resonant frequency and therefore its amplitude is changed by interaction with the surface. Since the behaviour of the system appears to be dependent on the gas pressure, it may be assumed that the coupling is due to hydrodynamic mteractions within the fork-air-sample gap. Since the fork tip-sample distance is approximately 200 pm -1.120), tire teclmique is sensitive to the near-field component of the scattered acoustic signal. 1 pm lateral and 10 mn vertical resolutions have been obtained by the SNAM. 

The main cost of this enlianced time resolution compared to fluorescence upconversion, however, is the aforementioned problem of time ordering of the photons that arrive from the pump and probe pulses. Wlien the probe pulse either precedes or trails the arrival of the pump pulse by a time interval that is significantly longer than the pulse duration, the action of the probe and pump pulses on the populations resident in the various resonant states is nnambiguous. When the pump and probe pulses temporally overlap in tlie sample, however, all possible time orderings of field-molecule interactions contribute to the response and complicate the interpretation. Double-sided Feymuan diagrams, which provide a pictorial view of the density matrix s time evolution under the action of the laser pulses, can be used to detenuine the various contributions to the sample response [125]. 

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