Replicative capacity

Antiviral Resistance and Impact on Viral Replication Capacity Evolution of Viruses Under Antiviral Pressure Occurs in Three Phases... 

Antiviral Resistance and Impact on Viral Replication Capacity... 

Fig. 1 Three phases in viral evolution during suboptimal therapy. The black hne represents wild type virus, whereas the red line represents mutant virus (Res stands for the level of resistance and RC for replication capacity).

The size of a cell bank is dependent upon the scale of operation, frequency of use and replicative capacity of the cell line in question. Typically, an MCB might contain 20-50 ampoules and a WCB 100-200 ampoules. Larger banks may be prepared for stable continuous cell lines and this is a major advantage of this type of cell line in comparison with finite cell lines of limited replicative capacity. 

Knippers, R., and W. Stratling. 1970. The DNA replicating capacity of isolated E. coli cell wall-membrane complexes. Nature (London), 226 713-717. 

Connor, R. I. and Ho, D. D. (1994) Human immunodeficiency virus type 1 variants with increased replicative capacity develop during the asymptomatic stage before disease progression. J. Virol. 68,4400-4408. 

Asjo, B., Morfeldt Manson, L., Albert, J., Biberfeld, G., Karlsson, A., Lidman, K., et al. (1986) Replicative capacity of human immunodeficiency virus from patients with varying severity of HIV infection. Lancet 2, 660-662. 

One profound implication of the specialized telomere structure and its synthesis is that in the absence of telomerase, the repeat length of telomeres could not be maintained. Telomerase is active in neonatal cells and also in some immortal tumor cells, but is barely detectable in diploid, terminally differentiated mammalian cells. Most such diploid cells can multiply in vitro in specialized culture medium, but have a limited life span. Loss of replicative capacity is associated with shortening of telomere repeat lengths. Furthermore, ectopic and stable expression of telomerase in human diploid cells by introduction of its gene confer an indefinite reproductive life on such cells. It... 

Tumell AS, Grand RJ, Gallimore PH. The replicative capacities of large ElB-null group A and group C adenovimses are independent of host cell p53 status. J Virol 1999 73 2074-2083. 

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