Reducing sugar production, measurement

A mycelial mat (2x2 mm ) was incubated in the medium for enzyme production in a 100-ml Erlenemyer flask for 5 days at 28°C using a rotary shaker. The amount of reducing sugar was measured using 3, 5-dinitrosalicylic acid. Two flasks were used for a strain. These values are average. 

The reducing sugar-based assays are predicated on the correct measurement of enzymegenerated product. A typical calibration curve for the BCA assay, using galacturonic acid as the calibration standard, is presented in Figure Cl.2.5. Note that the assay is linear for the... 

Polyamide microcrystalline powders form measurable polymer-bound fluorescent reaction products with malonaldehyde from oxidizing lipids and with reducing sugars. The compounds form on the terminal amine groups which appear to exist in zwitterionic fields with carboxylate anions, as revealed by titration with acid, alkali, or benzoquinones. 

Cellulase activities were determined on filtrates of culture samples by production of reducing sugar measured as glucose by a dinitrosalicylic acid (DNS) method (32) as follows ... 

Polarimetry The determination of sucrose and reducing sugars in fruit and fruit products by polarimetry is an AOAC recommended method. The polarimeter measures the rotation of plane polarized light caused by a solution containing an optically active compound. Separate measurements for the quantification of sucrose and reducing sugars can be made with the Clerget-Hertzfeld double-polarization method. 

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