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Reconstitution of apo-myoglobin

Semi-synthetic enzymes are produced by the reconstitution of apo-proteins with artificial active sites that yield novel catalytic functions [237]. For example, reconstitution of apo-myoglobin with Co(II)-protoporphyrin IX results in a novel biocatalyst that is capable of hydrogenating acetylene derivatives or evolving hydrogen [209, 238]. By the modification of the reconstitution of apo-proteins with artificial redox-active cofactors and the covalent attachment of photosensitizer units, photo-... [Pg.2557]

Figure 45. Reconstitution of apo-myoglobin with a Zn- protoporphyrin IX linked covalently to an electron acceptor (31 or 32) and vectorial photoinduced electron transfer in the assembly. Figure 45. Reconstitution of apo-myoglobin with a Zn- protoporphyrin IX linked covalently to an electron acceptor (31 or 32) and vectorial photoinduced electron transfer in the assembly.
Figure 3-1. The reconstitution of apo-myoglobin with functionalized heme units. Figure 3-1. The reconstitution of apo-myoglobin with functionalized heme units.
The reconstitution process is a well practiced methodology to examine the structure-function relationship of proteins. The reconstitution method. Fig. 3-1, (exemplified for myoglobin) involves the exclusion of the native active center from the protein, e.g. an ion or a cofactor, to yield the respective apo-protein (or hollow-protein). The implanting (or reconstitution) of a structurally-related cofactor or ion generates the semisynthetic reconstituted protein that might exhibit new tailored functions that are not present in the native protein. The reconstitution process has been extensively practiced upon studying mechanistic aspects of photoinduced ET... [Pg.39]

These studies prompted a reexamination of the stimulatoiy effects of apo cyt 65 in a reconstituted system with P450 3A4 and 17A1 [133], The reexamination concluded that far less heme transfer occurred than could be accounted for by the stimulatoiy effects of apo cyt by Furthermore, apo myoglobin did not inhibit the stimula-toiy effects of apo cyt by The reexamination did not include investigation of the effects of redox inactive cyt b which had been reconstituted with a Zn-substituted protoporphyrin IX. Nor did it investigate whether cyt b mutants that were unable to bind heme were still stimulatoiy. [Pg.59]


See other pages where Reconstitution of apo-myoglobin is mentioned: [Pg.39]    [Pg.40]    [Pg.40]    [Pg.39]    [Pg.40]    [Pg.40]    [Pg.194]    [Pg.297]    [Pg.2530]    [Pg.2558]    [Pg.2562]    [Pg.2563]    [Pg.2565]    [Pg.62]    [Pg.333]    [Pg.504]    [Pg.220]    [Pg.185]   
See also in sourсe #XX -- [ Pg.39 , Pg.40 ]




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