Recombination synthesis

A chemical library is a precisely defined collection of different chemical compounds. Chemical libraries can be either prepared by parallel synthesis or by split-and-recombine synthesis. 

These conceptual goals are attained by several combinatorial methods and tools. Characteristic for combinatorial chemistry is the synthesis on solid support or by polymer-supported synthesis, allowing for much higher efficiency in library production. Synthesis can be conducted either in automated parallel synthesis or by split-and-recombine synthesis. Centerpieces of combinatorial methods further include specific analytical methods for combinatorial... 

Split-and-Recombine Synthesis for the Preparation of Large Libraries... 

Introduced in the early 1990s, the split-and-recombine concept contributed much to the early success of combinatorial chemistry. Often, all combinatorial methods were identified with this concept. Split-and-recombine synthesis offered easy access to large number of individual compounds in few steps. If conducted on polymer beads, these are easily separated mechanically and can be identified subsequent to a screening step. 

Fig. 3.3. Principle of design of immunotoxin conjugates by chemical coupling of toxins, or through their recombinant synthesis as fusion proteins. The antibody components which are parts of the Fah fusion and single-chain fusion are shown in Fig. 3.4.

Furka and co-workers pioneered the split-pool synthesis method [19-22] for the synthesis of large peptide libraries in 1988 this approach is termed divide, couple, and recombine synthesis by other workers [44-47]. 

N-terminal protein ligation, an approach towards native protein ligation using split inteins. The protein of interest is obtained by ligation of a recombinant and a synthetic fragment. The C-terminal part is obtained by recombinant synthesis from a fusion protein containing the C-extein (C-terminal part of the target protein) and the... 

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