Terminal protein

Transcription continues into the AT region, and with the aid of the p termination protein the RNA polymerase stops, dissociates from the DNA template, and releases the nascent transcript. 

A nonsense codon may appear that would then result in the premature termination of amino acid incorporation into a peptide chain and the production of only a fragment of the intended protein molecule. The probabihty is high that a premamrely terminated protein molecule or peptide fragment will not function in its assigned role. 

In order to obtain a preliminary view on how the oligosaccharides may influence the structure about the N-terminus, the C-n.m.r. spectra of the N-terminal proteins of glycophorins and in various degrees of glycosylation were recorded see Figs. 7 and 8. 

Amphiphysin Nerve-terminal protein that associates with synaptic vesicles probably via AP2 bound to synaptotagmin. May function in endocytosis. 

Claros, M., Brunak, S., and von Heijne, G. (1997). Prediction of N-terminal protein sorting signals. Curr. Opin. Struct. Biol. 7, 394-398. 

Samyn B., Hardeman K., Van der Eycken J., and Van Beeumen J. (2000), Applicability of the alkylation chemistry for chemical C-terminal protein sequence analysis, Anal. Chem. 72, 1389-1399. 

The F-box protein family is the largest substrate-recognition subunit family. It enables the eukaryotic cells to use the SCF E3 machinery to ubiquitinate a large number of diverse protein substrates. So far, over 70 F-box proteins have been identified in the human genome [57, 58]. F-box proteins all share an 40-amino acid F-box motif, which is usually followed by a C-terminal protein-protein interaction domain such as the WD40 repeats j5-propeller (Fbw subfamily) and /eucine-rich repeats (LRRs Fbl subfamily Figure 7.5) [59, 60]. F-box proteins interact with... 

N-terminal methylation of proteins is possible and can play a major role in mitosis. Chen etal. discovered that the N-terminal serine or proline of regulator of chromatin condensation 1 (RCCl) is methylated. RCCl associates with chromatin, binding to histones H2 A and H2B, regulated by Ran, a GTPase. " For methylation to occur, the initiating methionine must be removed and a proline and lysine must be at positions 3 and 4, respectively. If RCCl is N-terminal methylation deficient, its binding to histones loses efficiency, which can cause spindle pole defects and ultimately cause problems in cell division. This work suggests the first known function for N-terminal protein methylation. ... 

The use of DNA sequencing as a method of generating protein sequence data (53) has proven to be extremely fast and efficient and future technical developments such as automated DNA sequencing will further expedite the production of protein sequence data (53). Nevertheless, DNA sequencing often calls for a certain amount of protein sequence information for the procedure to be most efficient. From our own experience in the amino terminal protein sequencing of over 500 samples, we find that approximately 50% of all naturally occurring proteins have ammo termini which are blocked, thus precluding their... 

Goldman, E. R., I. L. Medintz, A. Hayhurst, G. P. Anderson, J. M. Mauro, B. L Iverson, G. Georgiou, and H. Mattoussi. Self-assembled luminescent CdSe-ZnS quantum dot bioconjugates prepared using engineered poly-histidine terminated proteins. Anal. Chim. Acta 534, 63-67 (2005). 

Kainada, K., Iloriiiclii, T., Olisiimi, K., Shimamoto, N., Morikawa, K. (1996) Structure of a replication-terminator protein complexed with DNA. Nature 383, 598-603. 

C-Terminal protein (a protease inhibitor) Angiotensin I (10 residues DRVYIHPFHL)... 

Rho and other termination factors. Termination proteins can also react with specific regions of DNA or of an RNA transcript to terminate transcription.183 The best known termination factor is the rho protein a hexamer of 45-kDa subunits. It interacts with transcripts at specific termination sequences, which are often C-rich, and in a process accompanied by hydrolysis of ATP causes release of both RNA and the polymerase from the DNA.192193 Additional E. coli proteins, products of genes nus A and nus G, cooperate with the rho factor at some termination sequences.194-196c The rho hexamer is a helicase that moves along the RNA transcript in the 5 —> 3 direction driven by ATP hydrolysis. If it locates an appropriate termination signal, it may utilize its helicase activity to uncoil the DNA-RNA hybrid segment within the transcription bubble (Fig. 28-4).197 198b... 

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