Recognition, binding

Fig. 4.1 Topological organization of the vanilloid receptor TRP VI. Highlighted are the molecular determinants of TRPVl regulation, such as recognition (binding) domains for capsaicin and acids, and phosphorylation sites for protein kinases. Numbers designate the key amino acid residues deduced from the rTRPVl primary sequence. Adapted from Ferrer-Montaniel, A. et al. (2004) Fur. J. Biochem. 271, 1820—1826.

Ligand recognition/binding The carbohydrate content of antibodies, for example, plays a role in antibody binding to monocyte Fc receptors and interaction with complement component Clq... 

The cytoplasmic domain of the P-subunit displays three distinct sub-domains (a) the juxtam-embrane domain , implicated in recognition/binding of intracellular substrate molecules (b) the tyrosine kinase domain, which (upon receptor activation) displays tyrosine kinase activity (c) the C-terminal domain, whose exact function is less clear, although site-directed mutagenesis studies implicate it promoting insulin s mitogenic effects. 

FIGURE 5.17. Dynamics of molecular recognition. Binding of the target molecule to the receptor. Kinetic zone diagram and characteristic equations. Adapted from Figure 1 of reference 22, with permission from the American Chemical Society. 

FIGURE 5.18. Dynamics of molecular recognition. Binding of the target molecule to the receptor. Passage (left) from zone I to zone ID (a) and from zone I to zone IA (b). From left to right, log X — oo, 1.141, 0.5, 0 (a) — oo, —0.858, 0, 0.5 (b). Adapted from Figure 2 of reference 22, with permission from the American Chemical Society. 

Table 2 Bivalent Thrombin Inhibitors Interacting with both Fibrinopeptide and Fibrinogen Recognition Binding Sites...

Designed spacer covalently linking directing group and molecular recognition binding site... 

For the quantification of molecular recognition, binding constant K and binding free energy AG° are defined by (1) and (2), respectively. Since determination of K requires at least measurable concentrations of each component, K and AG° can only be accurately determined for ICs of types II-IV ... 

Molecular recognition, binding and transport of different chemical species represents an aspect of supramolecular chemistry that has relevance to a number of areas that include biochemical processes, analytical techniques, recycling and environmental processes as vell as aspects of catalysis and medicine. Over the years, a large number of both efficient and selective receptors for cations, anions, salts and zwitterions based on different architectures and binding modes have been developed and studied [1-4],... 

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