Semi-adherent cells

Some cell lines can proliferate as semi-adherent cells, and the population is a combination of suspended and adherent cells. To maintain the heterogeneous nature of such cultures, both suspended and adherent cells should be subcultured. 

Figure 6. Semi quantitative evaluation of spontaneous adsorption of collagen IV conjugated with a fluorescence marker Oregon Green 488 (A) to polyethylene foils iradiated with ions (energy 30 keV, dose from lO to lO ions/cm ), and its correlation with the number of rat aortic smooth muscle cells initially adhering to the polymer on day 1 after seeding (B). Collagen was diluted in phosphate-buffered saline to the concentration of 0.02 mg/ml (10 pg/cm ) and incubated with the foils for 24 h at room temperature. Mean+SEM from 4-9 experiments. Student t-test for unpaired data [43].

These swellings and markings seem to be due either to the insertion of other cells adherent to the fibre or to mechanical manipulations to which the fibres themselves have been subjected. Slight longitudinal striations are also present in some cases, but these do not prevent observation of the more pronounced middle line marking the channel. On the whole the fibres appear smooth and semi-transparent. Their ends are pointed. 

Inspect the cells on d 2, they should be semi-confluent and adherent to the flask base. Increase the volume of RPMI 1640/5% FBS medium to 75 mL and return to the incubator. 

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