Receptor-protein pair

Sensitive, selective detection of biochemically active compounds can be achieved by employing antigen-antibody, enzyme-substrate, and other receptor-protein pairs, several of which have been utilized in the development of piezoelectric immunoassay devices. The potential analytical uses of these materials has been reviewed, particularly with respect to the development of biochemical sensors [221-224], The receptor protein (e.g., enzyme, antibody) can be immobilized directly on the sensor surface, or it can be suspended in a suitable film or membrane. An example of the sensitivity and response range that can be... 

Another important application of cDNAs is to identify specific proteins in a tissue homogenate or tissue section. Since cDNAs undergo complementary base pairing, adding a radioactively labelled cDNA to a homogenate or tissue slice will bind it to the complementary sequence by a process of hybridization. Thus the amount of radioactive cDNA that hybridizes to the tissue or tissue extract is a measure of the amount of mRNA that is complementary to it. When this procedure is undertaken on slices of brain, it is known as in situ hybridization. In this way it is possible to determine the distribution of specific receptors in a tissue by accurately determining the distribution of mRNA that encodes for the receptor protein. This is a particularly valuable technique for the administration of psychotropic drugs. 

The response elements for glucocorticoids and estrogen receptors contain short palindromic sequences with various three-nucleotide "spacer" sequences in the center as follows.308,314,316-318 Two receptor proteins bind to the palindromic DNA forming a ho-modimeric receptor pair. For the 9-cis retinoic acid receptor RXR-a the response element contains a pair of direct repeats of a 6-base consensus sequence with a two-base pair spacer ... 

Atom-Pair Interaction Potentials. Affinities can be calculated based on ligand-receptor atom-pair interaction potentials that are statistical in nature rather than empirical. Muegge and Martin (320) derived these potentials from crystallographic data in the Protein... 

Current interpretations of ORN projections across taxa suggest that each ORN expresses just one type of receptor protein, and all ORNs expressing identical receptor proteins on their dendrites terminate within the same glomerulus. In mammals, each class of ORNs (about 1,000) which expresses the same receptor protein on its dendrites, terminates in a specific glomerulus, or pair of glomeruli, in the olfactory bulb (Munger et al. 2009) this principle, however, may not apply as strictly to insects or to other arthropods. 

---