Razor blade cutting

Using a single edge razor blade, cut thin (2-3 mm) slices of liver and place them immediately into Camoys fixative. 

Remove gel containing proteins of interest from the electrophoresis unit. Using a razor blade, cut the lower comer of the gel at the first lane. Equilibrate the gel in transfer buffer (see Basic Protocol 1, step 4). 

Take a plate of stripping film (12.1 X 16.5 cm) and cut it with a razor blade. Cut 1 cm inside and parallel to each edge and discard the narrow strips. Cut the central portion into eight squares... 

With a razor blade, cut a piece of rubber of about 1 to 2 g from your sample. 

With a single-edge razor blade, cut 2 to 3 mm-long sections of the suspect and scene fibers and place them on a clean sheet of paper out of a draft. Handle them with forceps. 

Fig. 4.4 Cryostat. Use a cryostat to cut sections from tissue frozen on a chuck, (a) The chamber of a cryostat with the frozen tissue indicated by the black arrow. This tissue was frozen in a rectangular mold, (b) The chuck with the tissue is placed in the arm. (c) The excess O.C.T. is trimmed off with a razor blade by cutting into the block several times, (d). The trimmed frozen O.C.T. is removed with a razor blade cut from the top of the block, (e) The knife is adjusted into position very close to the block, but not touching. The block is advanced toward the block until sections are cut. (f) The anti-roU plate prevents cut sections from curling, (g) An alternative to the anti-roll plate is to use a fine paint brush to hold a section as it comes off the block, (h) Sections are picked up on microscopes slides, (i) Lower the bottom of the slide until the section attaches then warm the slide, (j) When the section is dry, remove the dried film of O.C.T. arrows) with a forceps. Note the two rows of sections toward the slide label down) have the film already removed...

Vibratome - a device used on delicate, fixed tissue before sectioning a vibrating razor blade cuts large pieces of tissue into sections 25-500 p,m for freezing or processing. 

Using a knife or razor blade, cut off the match heads. 

Using two sharp razor blades cut tissue pieces of approx 2x2 mm from the region of the slice containing the cells of interest (see Notes 9 and 13). 

Using a razor blade, cut pieces of fused silica (FS) tubing, polyethylene (PE) tubing, and dialysis membrane to lengths of 16, 15, and 15 mm, respectively... 

Hearle et al. [4] described several special holders that are modifications of the metal plate method. Specimen holders which have holes for packing materials such as fibers, yams, or fabrics for razor blade cutting are useful methods which involve the insertion of a looped thread through the hole and the placement of the material in the loop. This material is then pulled through the hole and sectioned with a razor blade. Modifications to the stubs on any SEM can be made readily in a machine shop. 

A) Shear cutting B) Crush cutting C) Log cutting D) Razor blade cutting a) Web b) Log... 

In razor blade cutting (Fig. 18 D) the web is cut by parallel razor blades. The edges are cut very smoothly. Due to the lower stif iess of the blades the precision is lower than with the other methods. It is preferred for, e.g., carton-sealing tapes. 

Prepare 2.25% Bacto agar (Difco) in H O. Pour the agar into lOO-mm plastic petri dishes, approximately 7-mm thick, and cut 2.0-2.5-cm blocks with a razor blade. Cut a 45 bevel on the side on which the embryos will be aligned. This helps to view the embryos more clearly. 

Obtain a plastic bottle (an old bleach, shampoo, or beverage bottle will do). Using a razor blade, cut two cross sections through the length of the bottle at right angles to each other (one down the front, one down the side). Observe and explain any variation in thickness. 

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