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Random binding

We now turn attention to a mechanism where the sequence of substrate binding is not rigorously preserved, random binding. The formal kinetic scheme corresponding to Equation 11.37 is  [Pg.354]


Full and partial uncompetitive inhibitory mechanisms, (a) Reaction scheme for full uncompetitive inhibition indicates ordered binding of substrate and inhibitor to two mutually exclusive sites. The presence of inhibitor prevents release of product, (b) Lineweaver-Burk plot for full uncompetitive inhibition reveals a series of parallel lines and an increase in the 1/v axis intercept to infinity at infinitely high inhibitor concentrations. In this example, Ki = 3 iulM. (c) Replot of Lineweaver-Burk slopes from (b) is linear, confirming a full inhibitory mechanism, (d) Reaction scheme for partial uncompetitive inhibition indicates random binding of substrate and inhibitor to two mutually exclusive sites. The presence of inhibitor alters the rate of release of product (by a factor P) and the affinity of enzyme for substrate (by a factor a) to an identical degree, while the presence of substrate alters the affinity of enzyme for inhibitor by a. (e) Lineweaver-Burk plot for partial uncompetitive inhibition reveals a series of parallel lines and an increase in the 1/v axis intercept to a finite value at infinitely high inhibitor concentrations. In this example, Ki = 3 iulM and a = = 0.5. (f) Replot of Lineweaver-Burk slopes from (e) is hyperbolic, confirming a partial inhibitory mechanism... [Pg.122]

The catalytic subunit C of PKA consists of two domains, one composed mostly of a-helices and one of /3-strands, which are connected by a small hnker region. The ATP binding site is located deep in the active site between the two domains the binding site of the larger substrate is at the mouth of the pocket (Color Plate 4). A flexible activation loop is postulated to function as a door for the active site and is beheved to be directly involved in regulating PKA. PKA has a disordered or random binding mechanism. When the door is open, both the substrate and ATP have unhindered access to the active site and the binding of one does not influence the other. ... [Pg.347]

FIGURE 6-13 Common mechanisms for enzyme-catalyzed bisubstrate reactions, (a) The enzyme and both substrates come together to form a ternary complex. In ordered binding, substrate 1 must bind before substrate 2 can bind productively. In random binding, the substrates can bind in either order. [Pg.208]

Figure 1. A theoretical model for Na+-coupled solute transport. The model assumes a reversible system where only two forms of the carrier are mobile, the empty carrier, C, and the ternary complex, CSNa+. Neither CS nor CNa+ is mobile in either direction so that in absence of Na+ there is no translocation of S. In this model there is random binding of Na+ and S. The net direction of movement will be dictated by the direction of the driving forces. The external and internal milieux are represented by the symbols o and i, respectively. Figure 1. A theoretical model for Na+-coupled solute transport. The model assumes a reversible system where only two forms of the carrier are mobile, the empty carrier, C, and the ternary complex, CSNa+. Neither CS nor CNa+ is mobile in either direction so that in absence of Na+ there is no translocation of S. In this model there is random binding of Na+ and S. The net direction of movement will be dictated by the direction of the driving forces. The external and internal milieux are represented by the symbols o and i, respectively.
Rh(phi)2(phen)]3+ is a particularly suitable luminescence quencher for our investigations of electron-transfer reactions on DNA. Its electronic properties are favorable for electron transfer, and this rhodium complex is primarily sequence neutral, so that nearly random binding of the donor and acceptor is expected. Moreover, the photocleavage reaction actually allows us to identify the positions of binding of the acceptor to the DNA double helix. [Pg.458]

The catalytic pathway is best described as a random binding kinetic mechanism involving the formation of the ternary complex E-acetyl-P-ADP, with direct phosphoryl group transfer between enzyme-bound substrates to form the product ternary complex E-acetate-ATP. The formation and decomposition of these ternary complexes involve only noncovalent binding interactions of the enzyme with the substrates and products. The stereochemistry is inconsistent with a mechanism in which the phosphoryl group is transferred to an enzymic nucleophile as a step in the interconversion of the ternary complexes. The case of acetate kinase is one in which the stereochemical course of phosphoryl group transfer essentially discredited a double-displacement mechanism that had been reasonably well supported by other evidence. [Pg.161]

Recently, an examination of the binding kinetics of Rhodamine G-Iabeiled antibodies to dinitrophenol, also immobilized to a quartz light guide, was reported 91]. In a similar experiment, Rhodamine G-labelled IgG and insulin were shown to adsorb non-speciircally to serum albuminfused silica with both reversible and irreversible components. The characteristic time of the most rapidly reversible component measured was ca S ms and was limited by the rate of bulk diffusion. The binding was followed by fluorescence which, collected by a microscope from a ca S-pm surface area, spontaneously fluctuates as the solute molecules randomly bind to, unbind from, and/or diffuse along the surface in chemical equilibrium. [Pg.262]

Lang MC, de Murcia G, Mazen A, Fuchs RPP, Leng M, Daune M (1982) Non-random binding of N-acetoxy-N-2-acetylaminofluorene to chromatin subunits as visualized by immunoelectron microscopy. Chem Biol Interact 41 83-93... [Pg.234]

The amount of tRNA may not always be enough to outcompete the random binding of DNA or RNA. In particular, poly(rG) tends to interact with histones on the blots. Supplementation with herring sperm DNA and/or poly(dl)-poly(dC) DNA can eliminate this problem. We have successfully used both at concentrations ranging from 20 g/ml to 0.5 mg/ml. [Pg.344]

This is another type of accidental enzyme, showing that you can make a simple enzyme without really knowing what you re doing, so long as you can try enough possibilities and use iron s natural catalytic power. The same random bind a metal and let it do the work for you strategy works well for zinc and copper, because of their inherent stickiness and chemical power. [Pg.39]

The distinction between ordered and random binding of substrates during ternary complex formation is more difiicult and rarely absolute. For example the reaction of lactate dehydrogenase from pig heart muscle is classified as an enzyme reaction with an ordered mechanism in the reversible reaction... [Pg.93]


See other pages where Random binding is mentioned: [Pg.354]    [Pg.26]    [Pg.160]    [Pg.244]    [Pg.467]    [Pg.467]    [Pg.240]    [Pg.216]    [Pg.27]    [Pg.66]    [Pg.67]    [Pg.67]    [Pg.70]    [Pg.2174]    [Pg.5369]    [Pg.1809]    [Pg.465]    [Pg.396]    [Pg.467]    [Pg.467]    [Pg.210]    [Pg.22]    [Pg.122]    [Pg.296]    [Pg.355]    [Pg.465]    [Pg.2173]    [Pg.5368]    [Pg.161]    [Pg.19]    [Pg.286]    [Pg.304]    [Pg.155]    [Pg.35]    [Pg.427]   
See also in sourсe #XX -- [ Pg.354 ]




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