Radiolabelling technique

Chattopadhyay et al., 1992), and a comparison of radiolabeling techniques for the crosslinker (Shephard et al., 1988). Other studies have involved the investigation of protein interactions using the label transfer nature of radioiodinated SASD (Gupta et al., 2005 Lindersson et al., 2005 LeFebvre et al., 2006). 

The best radiolabeling technique for SASD is to use the Iodogen method (Shephard et al., 1988) described in Chapter 12, Section 3. The following suggested protocol for using SASD was based on the method described in the Thermo Fisher Catalog. 

The rapid turnover rate of some enzymes allows ELISAs to be designed that surpass the sensitivity of radiolabeling techniques. In addition, substrates can be chosen to produce soluble products that can be accurately quantified by their absorbance or fluorescence. Alternatively, substrates are available which form insoluble, highly colored precipitates, excellent for localizing antigens in blots, cells, or tissue sections. The flexibility of enzyme-based assay systems makes the chemistry of enzyme conjugation one of the most important application areas in bioconjugate techniques. 

Careful choice of the label and the conjugation chemistry is a key issue for detection and characterization of targeters. Among the quantitative methods, radiolabeling techniques are distinguished by their unrivaled sensitivity, but... 

To measure lung deposition by imaging, the aerosol must be first labelled or tagged with a suitable radionuclide. Radiolabelling techniques have been developed for current inhalation products including nebulizers, propellant-driven metered dose inhalers, and dry powder inhalers. 

Because natural samples often have background levels of organic material and the chemical substrate concentration is low, indirect measurement techniques can not be used and 14C, other radiolabelled techniques, or sensitive specific analytical methods are required. If radiolabelled methods are used, the cost of the method increases, due to the cost of synthesis of the radiolabelled material. Use of radiolabelled chemicals is required for registration studies on environmental fate testing of pesticides. 

The CRC thimble and glass fiber filter disks are suitable for use in radiolabeled techniques. With y-ray counting, the microfilters are placed in containers (for example, disposable cellulose acetate tubes) and sealed... 

Budesonide Development of TechneCoat (Pharmaceutical Profiles, Nottingham, U.K.) radiolabeling technique [39]... 

Because radiolabeling techniques are not precise and specific activities of the isotopes can vary, validation and calibration should be done for each radiolabeled aerosol produced. In particular, the measurement of emitted doses... 

Warren S, Taylor G, Smith J, Buck H, Parry-Billings M. Gamma scintigraphic evaluation of a novel budesonide dry powder inhaler using a validated radiolabeling technique. J Aerosol Med 2002 15(1) 15—25. 

Batch tests using radiolabeled techniques were employed for both the solubilization and biodegradation tests (33, 52). The soil-water suspensions used... 

Of the optical techniques that have become part of the analyst s armory, chemiluminescence is, perhaps, the latest to have come of age. Historically, absorbance spectrophotometry with chromogenic labels has offered the clinical analyst convenience and universality, since UV/visible spectrophotometers are both ubiquitous and amenable to automation. However, direct detection of chromophores without enzyme amplification is limited in sensitivity to 0.1-1.0 jiM, since few molar extinction coefficients are greater than 10. Radiolabel techniques do offer far superior sensitivity, but as the demands for highly sensitive analyses become more voluminous and more widespread in terms of their setting (i.e., both health-... 

RFLP, fluorescent sequencing technology SSCP analysis, radiolabelling techniques... 

The stmctural and conformational analysis of proteins adsorbed to solid surfaces is difficult because most common analytical methods are not compatible with the presence of the interacting solids. With recent developments in instrumentation and techniques, our understanding of protein adsorption behavior has improved considerably [4, 14]. The most commonly used techniques include attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), radiolabeling techniques, immunofluorescence enzyme-linked immunosorbent assay (ELISA), ellipsometry, circular dichroism (CD) spectroscopy, surface plasmon resonance (SPR), and amide HX with nuclear magnetic resonance (NMR). Atomic force microscopy (AFM) and scanning... 

We have used a radiolabelled technique for investigating further the production of prostacyclin by the human uterus (9) and have confirmed that the endometrium produced very little. Human myometrium can only produce small quantities of 6-oxo-PGF <, (although this is the major PG produced)... 

Mumby, S. M., and Buss, J. E. (1990) Metabolic radiolabeling techniques for identification of prenylated and fatty acylated proteins. In Methods A Companion to Methods in Enzymology (J. N. Abelson and M. I. Simon, eds.), Vol. 1, pp. 216-220. Academic Press, San Diego. 

---