Radio-labelling tritium

TLC-Radio-label Procedure — The volunteer subjects (described in Clinical Protocol) all received 100 yCi of tritium labeled A9-THC, along with the standard 4.0-5.0 mg intravenous dose. Two to three ml aliquots of plasma were analyzed by the procedure described by Wall (4). ... 

Nagarajan, S. Kellogg, M. S. DuBois, G. E. Williams, D. S. Gresk, C. J. Markos, C. S. Understanding the Mechanism of Sweet Taste Synthesis of Tritium Labeled Guanidineacetic Acid, J. Labelled Compounds and Radio-pharmaceut. 1992, 31, 599-607. 

Phosphors for radio-activation are used as mixtures with a ji-radioactive compound (e.g., 3H, 14C, or 147Pm compounds) whose radiation effects continuous luminescence of the phosphor. Tritium-labeled organic binders are used for the production of luminous products and paints. Because of the high cost of tritium, the phosphors must have a high efficiency. 

Tritium labelled compounds can also be used as true tracers for following the rates of homogeneous reactions. Thus, in the reaction of amines with epoxides, one can label the amine and by taking samples at fixed time intervals, quenching and subjecting them to radio-HPLC one can follow the disappearance of amine, the increase in the formation of a 1 1 complex and in some cases, the appearance of further complexes until reaction is complete (Fig. With so much information becoming available it is... 

Activity-based probes (ABPs) are tagged covalent and irreversible enzyme inhibitors. Formation of a stable covalent bond ensures that the inhibitor will remain attached to the polypeptide after protein denaturation, after which the tag (radio-isotope, biotin, fluorophore) allows visualization and/or identification of the thus modified enzyme or enzyme family. The first proteasome ABP described comprised a tritium-labeled lactacystin analog [6,7]. Proteasome bands are visualized on one-dimensional SDS-PAGE (sodium docecylsulfate-polyacrylamide gel electrophoresis) gel in an autoradiogram after treatment with a radiolabeled... 

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