Quantitative, polymerase chain reaction qPCR

Quantitative Polymerase Chain Reaction (QPCR) This method can determine the amount of DNA or RNA in a sample. Using the QPCR method, DNA can be amplified many times, allowing minute quantities to be assessed. 

Biodistribution studies are conducted to evaluate the dissemination and persistence of nucleic acid/DNA and viral vector-based vaccines. Vector persistence should be examined at more than one time point up to at least 21 days or more after the last injection 22 unpublished data) and the biodistribution and persistence of a plasmid is usually dependent upon the route of administration.20 These studies can be combined with a general toxicology study or may be conducted separately. In most cases, the DNA is extracted from the selected tissues and assessed by quantitative Polymerase Chain Reaction (qPCR).1516... 

The performance of the substrates was further evaluated by gene expression arrays and quantitative polymerase chain reaction (qPCR). Significantly higher expressions of osteoblast-specific genes for the DSQ50 pattern compared to cells cultured on a planar control could be detected. ... 

Real-time PCR or quantitative polymerase chain reaction (qPCR), is a highly sensitive technique that can be used to quantify genes of interest (functional... 

Haematology DNA damage in peripheral blood mononuclear cells and total hydrophilic antioxidant capacity in the plasma from 19 TB patients and healthy tuberculin test-positive controls were evaluated by single-cell gel electrophoresis and inducible isoform Nitric Oxide Synthase (iNOS) expression measured by quantitative polymerase chain reaction (qPCR). Compared to controls, pulmonary TB patients under treatment presented with increased DNA damage and increased antioxidant capacity, which diminished during treatment. TB patients showed lower iNOS expression, but expression tended to increase during treatment [36 ]. 

One of the most commonly used methods to detect host-cell DNA is quantitative polymerase chain reaction (qPCR), using random oligonucleotides (typically hexanucleotides) that will amplify the presence of low level host-cell genomic DNA fragments. This method may be used to detect and quantify host-cell DNA in in-process samples and the final drug substance. 

Quantitative polymerase chain reaction, also called real-time RT-PCR or QPCR, is a method which employs insertion of a signal, such as fluorescence or enzyme activity, into PCR products generated by RT-PCR to determine the amount of messenger RNA (mRNA) in a tissue accurately. 

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