Quantitative complement fixation

Antinucleotide sera have been tested also for binding of radiolabeled hapten-protein conjugates or labeled DNA and with quantitative complement fixation assays. Once characterized, specific serum or purified antibodies may be used also in immunohistochemical tests at the level of light or electron microscopy. 

M12. Mori, K. F., Immunoassay of follicle-stimulating hormone in human urine and serum by quantitative complement fixation. J. Endocrinol. 42, 55-63 (1968). 

Mehta et al, using the method of quantitative complement fixation, carried out extensive tests of cross-reactivity with monospecific rabbit antisera to human y and ju. chains (19). The IgG of each of six mammalian species tested cross-reacted with anti-human IgG. Monkey IgG... 

Cytotoxicity Cellular Qualitative or semi-quantitative Cell typing Complement fixation tests... 

Quantitative Micro Complement Fixation Serologic Properties of Pig Liver Carboxylesterase... 

A quantitative micro-complement fixation test and the hapten inhibition method have been used for determination of the antibody binding site on the chloram-... 

The complement system has the ability to combine irreversibly with antigen-antibody complexes. If the antigen is a protein component of the sheep erythrocyte cell surface, this combination induces lysis of the erythrocyte and thus offers an excellent quantitative indication of complement fixation. After incubation of the complement with the antigen-antibody complex under study, its fixation is studied by evaluating its residual haemolytic activity when the lytic antigen-antibody system is added. Since the concentrations of antibodies in the first system and of complement remain constant, haemolysis will be inversely proportional to the amoimt of antigen added to the incubation mixture (Table 1). 

Complement Fixation - Probably the most important practical application of complement research has been the develoimient of complement fixation tests for the quantitation of antigens and antibodies. Standard complement fixation tests are performed by mixing dilutions of cuntigen and antibody in the presence of a known amount of complement. After a suitable incubation period, erythrocytes sensitized with antibody (EA) are added. If coi l ent has not been fixed, the indicator cells (EA) will lyse fixation by the antigen-antibody complex will lead to a loss of hemolytic complement activity hence, no lysis of the indicator cells (details given in ref. 49). 

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