Complement fixation test

Symptoms next include dementia, tremor, and ataxia progressing most often to coma and death survivors show severe chronic meningoencephalitis. The American form of trypanosomiasis (Chagas disease) is a common health problem in Central and Southern America and is transmitted by reduviid bugs. Polymyositis, myocarditis and encephalomyelitis are constant features of the disorder but may be accompanied by more widespread visceral involvement. Infection can be confirmed by complement fixation tests but antibodies do not occur in the blood until about two months after infection. 

Cytotoxicity Cellular Qualitative or semi-quantitative Cell typing Complement fixation tests... 

Jules Bordet Physiology/Medicine Discovery of blood complement complement fixation test... 

The principle and the procedures for performing the micro complement fixation test have been presented in detail previously. Here, we record the use of micro complement fixation to measure the rates of dissociation and the equilibrium constants for dissociation of pig liver carbox-ylesterase (EC 3.1.1.1) as a function of pH and salt concentration. 

Fig. 27. Correlation of results of tests for parietal cell and IF antibodies in pernicious anemia patients, showing their independence. The parietal cell antibodies were tested by complement fixation test (CF) while the IF antibodies were assayed by electrophoretic retention test, i.e., immobilization of the intrinsic factor related Bj2 binder on electrophoresis. From Taylor et al. (T13).

M8. Markson, J. L., and Moore, J. M., Autoimmunity in pernicious anaemia and iron-deficiency anaemia. A complement fixation test using human gastric mucosa. Lancet ii, 1240-1243 (1962). 

In addition, SNOMED and LOINC are linked through a SNOMED code for each of the LOINC name parts, as illustrated in Figure 18-4. The SNOMED hierarchies for these codes wiE enable software systems to automatically compute that a complement fixation test for Rickettsia is a subtype of... 

The specificity of the complement-fixation system depends directly on the specificity of the antiserum, which should react exclusively with the gonadotropin being estimated. Butt and Lynch (B12) performed experiments designed to compare the specificity of a micro complement-fixation test with that of a radioimmunoassay for FSH. Their results demonstrated that their FSH antibody preparation cross-reacted with LH and HCG in the radioimmunoassay system, and could not be used because, when absorbed with these substances, it no longer reacted with FSH. However, with the same antiserum in the complement-fixation system, neither HCG nor LH fixed complement when present in concentrations ranging from 1 to 6000 ImU/ml, the maximum complement fixation occurring with 30 ImU FSH. It was concluded that their complement-fixation assay was specific for FSH. There are other examples in which complement fixation tests appear to be more specific than the radioimmunoassay systems (see Section 6.1.4.). This may arise from the fact that a system must be multivalent if complement is to be fixed, i.e., the antibody and antigen must have several complementary points... 

Antisera specific for FSH and LH are rare and have invariably been produced by chance. The specificity of such antisera varies depending on such factors as the purified material used as the antigen for testing and the types of assay method employed for the final determination. In general, it may be said that complement-fixation tests are more specific... 

Complement Fixation Test for Drug-Induced Thrombocytopenia... 

Most antigen-antibody complexes fix complement through the normal pathway. The technique of complement fixation has been largely applied to determine the titer of antibody (Lachman et al. 1973). The serum to be tested is first decomple-mented by heat at 56 "C for 40 min and diluted in small tubes. An equal amount of platelets, in the presence of suspected antigen, is added to each tube. Guinea pig complement is then added. This complement fixation test is more sensitive than the determination of platelet agglutinins and is now widely adopted. 

A quantitative micro-complement fixation test and the hapten inhibition method have been used for determination of the antibody binding site on the chloram-... 

From the results of immunodiffusion and complement fixation tests with antisera specific for each of the capsid polypeptides. 

Complement fixation test A complex serologic test used to detect small quantities of antibodies. 

The lesions seen in humans are indistinguishable for both orf virus and milker s nodule. They begin as a small red or reddish blue papule that enlarges to form a flat-topped hemorrhagic pustule, often crusted in its umbilicated center. Diagnosis is made by clinical observation and by means of the complement-fixation test, tissue culture, or electron microscopy. 

Techniques for the detection or assay of various substances based on the reaction of those substances with specific antibodies (or vice versa, i.e. the detection and assay of antibodies using antigens). Such techniques include agglutination reactions, automated immune precipitation, complement fixation tests, crossed electrophoresis, counter electrophoresis, double diffusion, enzyme immunoassay, fluoroimmunoassay, haem-agglutination, immunoelectrophoresis, immunofluoresence, radial immunodiffusion, spin immunoassay, immunofixation, immunoradiometric assay and radioimmunoassay. See separate entries for these subjects. 

An antibody acting against the thyroid microsomal fraction. It can be detected by complement fixation tests or immunofluorescence. 

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