Pulse-Field Gel Electrophoresis

Use of pulsed-field gel electrophoresis (PFGE) to separate large DNA fragments Permits isolation of large DNA fragments obtained by use of restriction endonucleases (rare cutters) that result in very limited cutting of DNA. 

Pulsed field gel electrophoresis, 9 746 Pulsed flashlamps, 14 619 Pulsed laser deposition chamber, 24 739 Pulsed laser deposition (PLD), 24 738-743 advancement of, 24 739 as an alternative deposition technique, 24 742-743... 

PCR is now the front-end process for other DNA profiling techniques such as pulse-field gel electrophoresis (PFGE), terminal restriction... 

A combination of pulse field gel electrophoresis to separate large DNA restriction fragments, amplification by PCR, and detection with p53 cDNA probes has been useful to study chromosomal deletions and p53 mutations in colorectal cancers (Bl). 

Fig. 7. Frequency distribution of plasma Lp(a) concentrations (a) and apo(a) alleles (b) in three populations. Plasma Lp(a) levels were measured in 381 subjects by the sandwich ELISA technique apo(a) allele size (number of K-4 repeats) was estimated using pulsed-field gel electrophoresis and genomic blotting. [With permission of Gaw et at. (G11).]...

Hennekinne, J.A., Kerouanton, A., Brisabois, A. and De Buyser, M.L., Discrimination of Staphylococcus aureus biotypes by pulsed-field gel electrophoresis of DNA macro-restriction fragments, J. Appl. Microbiol, 94, 321-329, 2003. 

Gregoire V, Beauduin M, Bruniaux M, et al. Radiosensitization of mouse sarcoma cells by fludarabine (F-ara-A) or gemcitabine (dFdC), two nucleoside analogues, is not mediated by an increased induction or a repair inhibition of DNA double-strand breaks as measured by pulsed-field gel electrophoresis. Int JRadiat Biol 1998 73(5) 511-520. 

Figure 4.8 Patterns of smaldigested DNA of Staphylococcus aureus isolates on pulsed-field gel electrophoresis (PFGE). The isolates were obtained from four patients in diversely distant locations within the United States. Lane 1 = ATCC29213 (positive control), lane 2 = a methi-cillin-resistant S. aureus isolated from a patient in Georgia lane 3 = a gylcopeptide intermediate S. aureus isolated from a patient in Michigan, lane 4 = a methicillin-resistant S. aureus from the same patient in Michigan, and lane 5 = a glycopeptidet-intermediate S. aureus isolate from a patient in New Jersey. (From Smith et al., 1999b.)...

Zadoks R., W. van Leeuwen, H. Berkema, O. Sampimon, H. Verbrugh, Y.H. Schukken, and A. van Belkum (2000). Application of pulsed-field gel electrophoresis and binary typing as tools in veterinary clinical microbiology and molecular epidermiologic analysis of... 

Figure 5-42 Intact DNA from the chromosomes of three strains of the malaria parasite Plasmodium falciparum, ranging from 750 Kb to 5 Mb, separated by pulsed-field gel electrophoresis. Courtesy of C. Smith and T. E. Wellems. Reproduced by permission of Amersham Pharmacia Biotech Inc.

C. Bustamante, S. Gurrieri, and S. Smith, Tibtecb 11, 23-30 (1993). Towards a Molecular Description of Pulsed-Field Gel Electrophoresis. ... 

Cantor, C. R., C. L. Smith, and M. K. Mathew, Pulsed-field gel electrophoresis of very large molecules. Ann. Rev. Bio-phys. Chem. 17 287-304, 1988. 

Schwartz and Cantor developed pulsed field gel electrophoresis for the separation of very large DNA molecules. 

Storb, U., Haasch, D., Arp, B., Sanchez, P., Cazenave, P.-A., Miller, J. (1989). Physical linkage of mouse X genes by pulsed-field gel electrophoresis suggests that the rearrangement process favors proximate target sequences. Mol. Cell. Biol. 9,711-718. 

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