Pseudomonas fiuorescens

Eernandez RE et al. (2004) Enzymatic assimilation of cyanide via pterin-dependent oxygenolytic cleavage to ammonia and formate in Pseudomonas fiuorescens NCIMB 11764. Appl Environ Microbiol 70 121-128. 

Cellulomonas fimi Endoglucanase A 11) Cellulomonas fimi Exoglucanase (29) Microbispora bispora Endoglucanase I (5) Pseudomonas fluorescens CMCase (22) Pseudomonas fiuorescens Xylanase A 30)... 

Amide hydrolysis is carried out in water and requires some solubility of the substrate, while amide synthesis is carried out in a suitable solvent with an acylating reagent. The substrates are most commonly primary amines, with few reports of secondary amines [5]. The acylating reagent is selected to be unreactive toward the amine in the absence of the enzyme, but must be compatible with the enzyme providing acceptable rates. Enzymes that selectively hydrolyze or acylate the (R) amide or primary amine are most common, for example. Pseudomonas fiuorescens... 

Alanine racemases are homodimeric enzymes of an apparent molecular mass of 76kDa, containing two molecules of PLP as co-enzyme [61] the known enzymes from different sources are highly homologous. With regard to the kinetic properties, the values for D- and L-Ala determined at 30 °C for the Pseudomonas fiuorescens alanine racemase are -12 and 19mM, respectively and the Vnm values for racemization are -1200 and 2200 units/mg protein, respectively. The thermophilic alanine racemase from B. stearothermophilus is quite stable to heat treatment (up to 75 °C for 1 h) while the mesophilic one from B. subtilis is stable up to 55 °C under the same conditions. 

Pseudomonas denitrifieans, nitrite reductase of, 274-275 Pseudomonas fiuorescens transhydrogenase of, 52-53 purification, 54, 56... 

Nicotinamide nucleotide transhydrogenase was originally discovered in Pseudomonas fiuorescens. Part of the work done with these bacteria by Kaplan and co-workers (see 7) appeared later to have involved Pseudomonas aeruginosa. There is little doubt, however, that these two strains contain transhydrogenases that are closely related. Kaplan and co-workers (S) also demonstrated the presence of transhydrogenase in... 

Pseudomonas fiuorescens 200 11.5 16 Devoid of various reductase and dehydrogenase activities tested 1... 

The lipase-catalyzed polymerization of the disubstituted TMC analog 5-methyl-5-benzyloxycarbonyl-l,3-dioxan-2-one (MBC) was also studied [89]. The bulk polymerization, catalyzed by Pseudomonas fiuorescens lipase for 72 h at 80 °C, gave 97% monomer conversion and product in 97% yield with M = 6100. The benzyl... 

Protected glycerol monoacetate 19 can be prepared in acceptable enantiomeric purity and yield by crude pig pancreas lipase or Pseudomonas fiuorescens lipase-... 

Fig. 2. Tryptophol oxidation catalyzed by tryptophan side-chain oxidase from Pseudomonas fiuorescens...

Rustler, S., Motejadded, H., Altenbuchner, Jand Stolz, A. (2008) Simultaneous expression of an arylace-tonitrilase from Pseudomonas fiuorescens and a (S)-oxynitrilase from Manihot escu-lento in Pichia pastoris for the synthesis of (S)-mandelic acid. Appl. Microbiol. Biotechnol, 80, 87-97. 

Rustler, S., Muller, A., Windeisen, V., Chmura, A., Fernandes, B.C.M., Kiziak, C., and Stolz, A. (2007) Conversion of mandelonitrile and phenylglycinenitrile by recombinant E. coli cells synthesizing a nitrilase from Pseudomonas fiuorescens EBC191. Enzyme Microh. Technol., 40, 598-606. 

The demonstration that urocanic acid was an intermediate in the conversion of histidine and proof of the reaction pathway to glutamic and formic acids was first established in cell-free extracts of Pseudomonas fiuorescens. 

Protocatechuic acid is an intermediate in the metabolism of p-hydroxybenzoic acid by Vibrio species and by Pseudomonas fiuorescens (235,675,694,695). From the enzymically active dried cells (694) a protocatechuic acid oxidase has been extracted and partially purified... 

Keller S, Wage T, Hohaus K, Holzer M, Eichhorn E, Van Pee K-H. Purification and partial characterization of tryptophan 7-halogenase (PmA) from Pseudomonas fiuorescens. Angew. Chem. Int. Ed. 2000 39 2300-2302. 

Toxicoiogy LD50 (orai, rats) 2 g/kg irritating to eyes, skin Environmentai LC50 (brachydanio rerio, 48 h) > 1-10 mg/i EC50 (pseudomonas fiuorescens) 1 g/i up to 99% biodeg,... 

Numerous bacteria have been found to synthesize nicotinic acid in pure media. E. colt, B, aerogenes, A. fecalis, B. vulgalus, B, mesentericus (60), Aerobacter aerogenes, Pseudomonas fiuorescens, Proteus vulgaris, and Clostridium butylicum (65). 

Shortly after the identification of pantothenic acid with the chick antidermatitis factor (96) and as a necessary vitamin for the rat (97), evidence for its synthesis was reported in the rumen of ewes (49), cows (68), and calves (51). This synthesis is evidently appreciable since the pantothenic acid output in milk alone was about twice the intake (68). Pseudomonas fiuorescens, Proteus vulgaris, and Clostridium butylicum were found to synthesize pantothenic acid in a pure medium (65). 

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