Pseudomonas alkaline protease

Boumann, U., et al. Three-dimensional structure of the alkaline protease of Pseudomonas aeruginosa, a two-domain protein with a calcium binding parallel beta roll motif. EMBO J. 12 3357-3364, 1993. 

The first structures of this kind were reported in 1993 pectate lyase G from Erwinia chrysanthemi (Yoder et al, 1993) and alkaline protease from Pseudomonas aeruginosa (Baumann et al, 1993). Based on consideration of these crystal structures, the term parallel //-helix was introduced for a fold containing three //-strands per coil, and parallel //-roll for a fold with two //-strands per coil (Baumann etal, 1993 Yoder andjurnak, 1995 Yoder et al., 1993). The epithet parallel was intended to emphasize the distinction between these folds and the previously observed helical structure of the antibiotic gramicidin which contains both l- and D-amino acids and... 

H 0 A-butanoyl-L-homoserine lactone, BHL or C4-HSL Aeromomas hydrophila Aeromonas salmonicida Pseudomonas aeruginosa, Serratia liquefaciens Extracellular protease, biofilm formation. Extracellular protease. Virulence factors - alkaline protease, cyanide, elastase, haemolysin, lectins, pyocyanin, rhaminolipid, RpoS Swarming, protease. 

A r-(3-oxododccanoyl)-L-homoscrinc lactone, OdDHL or 30, C12-HSL Pseudomonas aeruginosa Virulence factors - alkaline protease, clatasc, exotoxin A, haemolysin, neuraminidase, exoenzyme S, Xcp secretion, RhlR, biofilm formation. 

Walasek, P. and Honek, J. F. (2005) Nonnatural amino acid incorporation into the methionine 214 position of the metzincin Pseudomonas aeruginosa alkaline protease. BMC Biochem., 6, 21. 

Louis, D., Kohlmann, M. and Wallach, J. (1997) Spectrophotometric assay for amidolytic activity of alkaline protease from Pseudomonas aeruginosa. Anal. Chim. Acta, 345, 219-225. 

Similar to Figure 1, the palmitoyl (C-16) group has also been enzymatically grafted onto HEC. The reaction entails the incubation of HEC, vinyl palmitate, and a suitable enzyme in N,N-dimethylacetamide (DMAc) at 50°C. Several enzymes were attempted satisfactory results were obtained with Pseudomonas fluorescens lipase (Amano P-30), Pseudomonas capecia lipase (Lipase PS), or Alcalase immobilized alkaline protease from Novozymes A/S as a catalyst. Note that in this case a polar aprotic solvent (DMAc) is used, and one of the reactants, vinyl palmitate, has a fricile leaving group (vinyl alcohol which leaves as acetaldehyde). 

Bhoopathy, N. R., Indhuja, D., Shrinivasan, K., Uthirappan, M., Gupta, R., Ramudu, K. N., Chellan, R. Statistical medium optimization of an alkaline protease from Pseudomonas aeruginosa MTCC 10501, its characterization and apphcation in leather processing. Indian J Expt Biol 2013, 51, 336-342. 

Guzzo, J., Pages, J. M., Duong, R, Lazdunski, A., Murgier, M. Pseudomonas aeruginosa alkaline protease evidence for secretion genes and study of secretion mechanism. J Bacterial 1991,173, 5290-5297. 

Kulkarni N, Gadre RV (1999) A novel alkaline, thermostable, protease-free lipase from Pseudomonas sp. Biotechnol Lett 21 897-899... 

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