Proteomics-Human diseases

APPLICATIONS OF PROTEOMICS I PROTEOMICS, HUMAN DISEASE, AND MEDICINE... 

JUNGBLUT, P.R., ZIMMY-ARNDT, U, ZEINDL-EBERHART, E STULIK, J., KOUPILOVA, K., PLEISSNER, K.P., OTTO, A., MULLER, E.C., SOKOLOWSKA-KOHLER, W., GRABHER, G., STOFFLER, G., Proteomics in human disease Cancer, heart and infectious disease, Electrophoresis, 1999, 20, 2100-2110. 

Jungblut, P. R., Zimny-Arndt, LF., Zelndl-Eberhart, E., Stuhk, J., Koupllova, K., Pleissner, K. P., Otto, A., Muller, E. C., Sokolowska-Kohler, W., Grabber, G., and Stoffler, G. (1999). Proteomics in human disease. Cancer, heart, and infectious diseases. Electrophoresis 20, 2100—2110. 

In the recent literature, many examples of A/BPs containing benzophenones can be found. A first example concerns the study of HDACs. These enzymes catalyze the hydrolysis of acetylated lysine amine side chains in histones and are thus involved in the regulation of gene expression. There are approximately 20 human HDACs, which are divided into three classes (I, II, and III). Class I and II HDACs are zinc-dependent metallohydrolases that do not form a covalent bond with their substrates during their catalytic process, which is similar to MMPs. It has been found that hydroxamate 65 (SAHA, see Fig. 5) is a potent reversible inhibitor of class I and II HDACs. In 2007, Cravatt and coworkers reported the transformation of SAHA into an A/BP by installment of a benzophenone and an alkyne moiety, which resulted in SAHA-BPyne (66) [73]. They showed that the probe can be used for the covalent modification and enrichment of several class I and class II HDACs from complex proteomes in an activity-dependent manner. In addition, they identified several HDAC-associated proteins, possibly arising from the tight interaction with HDACs. Also, the probe was used to measure differences in HDAC content in human disease models. Later they reported the construction of a library of related probes and studied the differences in HDAC labeling [74], Their most... 

The broad use of proteomic studies and microarrays seems to prove that such diagnostic tests are useful in medicine and research on allergens, allergies, and other human diseases (Ellmark et al., 2008 Person et al., 2008). Likewise, miniaturization of analytical instruments, which profoundly enhances research potential, stimulates the development of these methods. 

In 2001, the German Federal Ministry of Education and Research (BMBF) initiated the National Genome Research Network (NGFN) as a nation-wide multidisciplinary platform network aiming at the analysis of common human diseases, as well as aging. Within the NGFN the so-called Human Brain Proteome Project (HBPP) focuses on the analysis of the human brain in health and disease. The concept is based on three consecutive steps ... 

Appropriate animal models of human heart disease are an attractive alternative for proteomic investigations as human diseased tissue samples can often be compromised by factors such as the disease stage, tissue heterogeneity, genetic variability, and the patient s medical history/therapy. Avoiding any of the above complications when working with human samples can prove to be extremely difficult... 

Human Disease Glycomics/Proteome Initiative (HGPI) (Japan)... 

---