Proteomic technologies illustration

Fig. 2 Diagram illustrating the key proteomic technologies and the direction of flow from one technology to the next.

Established proteomic technologies, together with the new and alternative strategies currently under development, are now making it possible to address important issues in biomedicine. In this chapter we have attempted to illustrate how proteomics is being used to characterise protein... 

Proteomics research is currently driving HPLC technologies towards high sensitivity, speed, and peak capacities.8 Capillary LC (sometime termed micro LC or nano LC)18,19 is used to enhance mass sensitivity needed to analyze minute sample amounts. System requirements for handling extremely low flow rates and small peak volumes mandate the use of specialized HPLC system such as the one shown in Figure 4.14a, which has a micro-pump, a micro x-y-z autosampler, and a PDA detector with a low-volume flow cell. Figure 4.14b illustrates the performance at 12pL/min of this system. 

The purpose of this review is to survey the state of chemical genomics and proteomics at the end of the first decade of the twenty-first century, some 20 years after the names were first coined. Some examples are given to illustrate how chemical omic technologies are being used to address problems of current biological and clinical interest. 

---