Yeast mitochondrial DNA

Figure 5-48 A physical map of the oxi3 locus of yeast mitochondrial DNA. The restriction fragments used for DNA sequencing are indicated by the arrows. The extent to which the sequences were read is represented by the lengths of the arrows. The map units are shown in the inner circle. The following symbols, together with the names of the restriction enzymes (Chapter 26), are used for the restriction sites ...

Figure 1.3. A. The percentages of the four nucleotides, A (circles), G (squares), C (diamonds) and T (triangles) in the 3 -terminal, 5 -terminal and 5 -penultimate nucleotides formed by the spleen and the snail DNase from bacterial DNAs (Haemophilus influenzae, 38% GC Escherichia coli, 51% GC Micrococcus luteus 72% GC) are plotted against the GC level of DNAs. Values obtained at an average chain length of 15 nucleotides were used. B. Deviation patterns of three repetitive DNAs. The histograms show the differences between the composition of termini formed from guinea pig satellite, mouse satellite and yeast mitochondrial DNAs by spleen and snail DNase and the compositions expected for bacterial DNAs having the same GC level tl, terminal pt, penultimate. (From Bernardi et al., 1973).

Beilharz M.W., Cobon G.S., Nagley P. (1982). Physiological alteration of the pattern of transcription of the oU2 region of yeast mitochondrial DNA. FEBS Letters 147 235-238. 

Bernardi G. and TimashefFS.N. (1970). Optical rotatory dispersion and circular dichroism properties of yeast mitochondrial DNA s. J. Mol. Biol. 48 43-52. 

Blanc H. and Dujon B. (1980). Replicator regions of the yeast mitochondrial DNA responsible for suppressiveness. Proc. Natl. Acad. Sci. USA 77 3942-3946. 

Coruzzi G., Bonitz S.G., Thalenfeld B.E., Tzagoloff A. (1981). Assembly of the mitochondrial membrane system. Analysis of the nucleotide sequence and transcripts in the oxil region of yeast mitochondrial DNA. J. Biol. Chem. 256 12780-12787. 

Hudspeth M.E., Ainley W.M., Shumard D.S., Butow R.A., Grossman L.l. (1982). Location and structure of the varl gene on yeast mitochondrial DNA nucleotide sequence of the 40.0 allele. Cell 30 617-626. 

Yeast mitochondrial DNA occurs as double-stranded 26-/im closed circles, a molecular size corresponding to about 50 x 10 daltons. The number of circles per mitochondrion may range from zero to about five the total amount of cellular mtDNA per wild-type cell varies with the strain and accounts for 10-25% of the total cellular DNA. RNA-DNA hybridization studies indicate that yeast mtDNA contains one cistron of each of the 15 S and 21 S RNA species and probably 20 tRNA cis-trons. It has been reported that mitochondria from HeLa cells contain only 12 tRNA cistrons, 9 on the heavy DNA strand and 3 on the light strand. These authors suggested that since the proteins formed by mitochondrial ribosomes are enriched in hydrophobic amino acids, an array of 12 tRNAs may be sufficient for the complete synthesis of the inner-membrane proteins by mitochondria. Alternatively, some nuclear coded tRNAs may be available to the mitochondrial protein-synthesizing system. 

Animal DNA Allergen checker [Animal] Oriental Yeast Co., Ltd. 16S rRNA gene of mitochondrial DNA 370 70... 

Molecular techniques have enabled the identification of up to six types of flor yeasts according to electrophoretic karyotype and mitochondrial DNA restriction patterns (Ibeas et al. 1997). However, yeast types distribute in a non-uniform manner... 

Cho, J.H., Lee, Y.K. and Chae, C.B. (2001) The modulation of the biological activities of mitochondrial histone Abf2p by yeast PKA and its possible role in the regulation of mitochondrial DNA content during glucose repression. Biochim. Biophys. Acta 1522, 175-186. 

The subunits of FgFi in eukaryotic cells are coded partly by nuclear and partly by organellar genes. Mitochondrial DNA of human cells contains only the gene for subunit 6 [28], but that of yeast cells contains genes for both subunits 6 and 9 of MFq [27,62,63], corresponding to subunits a and c of EFg, respectively. Chloroplast... 

Although a few subunits of mitochondrial membrane proteins are coded by mitochondrial DNA and synthesized in the mitochondrial matrix, most membrane proteins including the adenine nucleotide carrier are coded by nuclear genes and synthesized on cytoplasmic ribosomes [80,81], Chloramphenicol, an inhibitor of mitochondrial protein synthesis, does not inhibit incorporation of radioactive leucine into the carrier in growing Neurospora crassa, but cycloheximide, an inhibitor of cytoplasmic protein synthesis, does inhibit leucine incorporation [78]. Also, a yeast nuclear respiratory mutant has been shown to cause a defect in adenine nucleotide transport [81], and the nuclear gene responsible for coding the carrier in yeast is currently being cloned for further studies [82]. 

---