Proteins on solids

A large number of potential applications for organized protein monolayers have recently motivated considerable research activity in this field (Boussaad et al. 1998, Kiselyova et al. 1999). Construction of specific interaction-directed, self-assembled protein films has been performed at the air-water interface. The Langmuir-Blodgett (LB) technique has been extensively used to order and immobilize natural proteins on solid surfaces (Tronin et... 

Immobilization of biomolecules and the assembly of biomolecules in defined arrays is a desired requirement of nanobiotechnology. Thin films of immobilized proteins on solid supports are of growing interest and find applications in biosensors, information processing, optics, and biomedicine [113-115],... 

Most often used for the detection of proteins on solid matrices such as polyacrylamide gels. 

Only the methods used in this study (i, 2, 3) can detect and follow the initial events at the boundary between a solid substrate and the biological milieu. This extraordinary sensitivity derives from the ability to monitor events from within the substrate itself by making the substrate capable of supporting multiple attenuated internal reflection (MAIR) at a variety of useful spectroscopic wavelengths. In other studies, it was concluded that adsorbed protein on solid substrates is of essentially native i.e. solution or volume phase) conflguration and present in signiflcant thickness (6). Those studies used a different version of the internal reflection spectroscopic method wherein a prism element was forcefully... 

We beheve that this approach of handhng membrane proteins on solid supports constitutes a promising route for analytical strategies aimed at analysis of membrane proteins and consequently also for the QCM-D technique in the development of patterned surfaces for array-based analysis of biorecognition events. 

Claudia Steinem, Andreas Janshoff Specific Adsorption of Proteins on Solid Supported Membranes. 

Immobilized metal ion-affinily chromatography (IMAC) was first established as a technique to fractionate proteins on solid supports based on their differential affinity towards immobilized metal ions. This differential affinity derives from the eoordination bonds formed between metal ions and certain amino acid side ehains exposed on the surface of the protein molecules. Since the interaetion between the immobilized metal ions and the side chains of amino acids has a readily reversible character, it can be used for adsorption and then be disrupted under mild conditions, usually by adding a competing agent. ° The use of this technique to purify recombinant proteins containing a short affinity-tag consisting of poly-histidine residues represent its main application (the most widespread and versatile strategy used to purify recombinant proteins). ... 

Molecular Orientation of Proteins on Solid Substrates. Cytochrome c... 

In spite of these murky aspects, which concern the adventures in the field of syntheses of polypeptides and proteins on solid phase by incremental chain elongation, the method of Merrifield is invaluable in the quick and convenient preparation of smaller peptides... 

Nakanishi K. Sakiyama T. Imamura K. On the adsorption of proteins on solid surfaces, a common but voy armplicated phenomenoa Journal of Bioscience and Bioengineering 2001 91(3) 233-44. 

Proteomics In MIcrotIuldic Devices, Figure 1 (aHf) Several heterogeneous protein assay formats (reprinted from [2]). A variety of protein binding events can be studied, ranging from antibody/antigen to protein/protein to enzyme/substrate interactions, (g) Strategies for the immobilization of proteins on solid supports (reprinted from [4])... 

Therefore, an understanding of the conformational behaviour of proteins at solid-solution interfaces is desirable for a variety of reasons. For example, a detailed mapping of conformational changes is necessary for understanding the mechanism of protein adsorption and can help identify optimal conditions to preserve functionality following protein immobilization. Even though major scientific contributions have been made to our understanding of proteins on solid surfaces in recent years... 

The adsorption of proteins on solid surfaces is an important phenomenon taking place as soon as a foreign material is brought into contact with a biological system. It is thus involved in situations of bio- and blood compatibility and in fouling in the process industry. Furthermore, there are several surface orientated diagnostic methods based on protein adsorption and interaction on solid surfaces. Proteins are large and complicated molecules and the adsorption process is therefore far from simple to model. 

Retention, Unfolding, and Deformation of Soluble Proteins on Solids... 

The use of nanoparticles for micropatteming of proteins on solid substrate has also been demonstrated [110]. Micropatteming of proteins can be achieved by immobilizing proteins on silica nanoparticles followed by self-assembly of the nanoparticles in patterned nucrowells (Figure 14.6). Because the placement of... 

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