Proteins, immobilization onto functionalized

Capture array involves the immobilization of non-protein molecules onto the surface which can interact with proteins in the solute phase. Generally, capture molecules may be broad capture agents based on chromatography type surface chemistries such as ion exchange, hydrophobic and metal affinity functionality, or they may be highly specific such as molecular imprinted polymers or oligonucleotide aptamers. 

Immunoadsorption, an advanced therapeutic modality, focuses on detoxification of patient blood rich in high-molecular-weight pathogenic substances, mostly abnormal autoantibodies such as rheumatoid factors in rheumatoid arthritis (RA) and anti-DNA autoantibodies in systemic lupus erythematosus (SLE). Detoxification of these pathogens will be accomplished through extracorporeal perfusion of the patient plasma or whole blood over an affinity column made of immunoadsorbents. These adsorbents perform their function through the same mechanism as conventional affinity adsorption, where proteins in the liquid phase are adsorbed on the specific ligands immobilized onto an insoluble support. 

FIGURE 40 Effect of changes in the feed superficial velocity on the experimental breakthrough curve for the adsorption of HEWL to Cibacron Blue F3G-A immobilized onto Fractosil 1000 as a function of time and the amount of protein added to the column. Data adapted from Ref. 436. 

Immobilization of Proteins and Enzymes onto Functionalized Polypropylene Surfaces by a Gaseous Plasma Modification Technique... 

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