Proteins biological assembly

Kentsis, A., Borden, K.L.B. (2004). Physical mechanisms and biological significance of supramolecular protein self-assembly. Current Protein and Peptide Science, 5, 125-134. 

In living cells, proteins are assembled from amino acids at a very high rate. For example, E. coli cells can make a complete, biologically active protein molecule containing 100 amino acid residues in about 5 seconds at 37 °C. How does such a polypeptide chain arrive at its native conformation Let s assume conservatively that each of the amino acid residues could take up 10 dif-... 

Since photoisomerization involves motion of one-half of the probe molecule against the other, it remains unaffected by the overall motions of the organized assemblies, such as the motion of different segments of DNA, or of the peptide chains of a protein or the surfactants in a micelle. In contrast, in optical anisotropy studies, the bending and twisting motion of the macromolecular chains of DNA, proteins, or micelles become superimposed on the orientational motion of the probe. As a result, photoisomerization seems to be a better probe for the microviscosity of complex biological assemblies. 

Spremulli L (2001) Protein synthesis, assembly and degradation. In Buchanan BB, Gruissem W, Jones RL (eds.) Biochemistry and Molecular Biology of Plants, p. 412. Am. Soc. Plant Physiologists, Rockville, MD, USA... 

There is also a similarity in the folding topology of the plant lectin concanavalin-A and VP1 (Argos, Tsukihara and Rossmann 1980). Hence, it is possible that the corresponding gene for a protein such as conA (figure 3.8) provided a useful tool for the formation of biological assemblies that could attach themselves to animal cell receptors. 

All of the chapters have been extensively revised since the 4th edition appeared in 2002. We have not attempted to describe in detail the structure and function of biological membranes or the mechanism by which proteins are assembled into membranes as these topics are covered elsewhere in a number of excellent books. The first chapter, however, contains an up-to-date summary of the principles of membrane structure as a basis for the material covered in the subsequent chapters. 

The design of new proteins that can assemble into filaments, symmetric cages, and regular assays was described by Yeates and Padilla.These investigations are based on the consideration that proteins self-assemble to fulfill their biological functions and also as part of a... 

Specific mode shape patterns are formed and components are forced to move to nodes and antinodes at resonant frequencies of the droplets and structures on which the droplets are placed. Microgels and microbeads of various sizes in a droplet were successfully assembled by excitation at various frequencies and intensities. The control over individual components is not possible however, a high number of components can be robustly manipulated. Both single- and double-layer structures (with a second assembly on top of the first assembly) were reported. The hydrogels used in the study are capable of encapsulating cells and proteins to create biological assemblies. 

Many important systems and processes, especially biological assemblies such as cell walls and protein secondary and tertiary structures form as a result of physical, inter- and intramolecular interactions. Such assemblies and... 

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