Proteins amino-acid sequencing

DNA base sequence —> RNA base sequence —> protein amino acid sequence... 

Protein (amino acid) sequences are available from databases such as SwissProt/ Trembl or Protein Data Bank (PDB). Most useful is the ability of such databases to perform alignments, the comparison between different sequences. Simple alignment compares two sequences, multiple alignment more than two. 

Using rational metabolic engineering to tailor the stereoselectivity of yeast-mediated reductions is feasible only when all of the relevant genes are known and the relationships between enzyme and acceptable substrates are defined. We are far from this ideal. For a few reductase proteins, amino acid sequence data has... 

Pyrolysis studies on proteins have been done for a variety of purposes. One of these is to obtain information on the primary structure of proteins (amino acid sequence) [1]. 

It is the sequence of amino acids, referred to as the primary structure of the protein, that determines the native conformation, the structure that is stable under physiological conditions. The first protein amino acid sequence was determined by F. Sanger and coworker in 1953 for insulin.54 It is generally believed that the native structure corresponds to a free-energy minimum, although there is no direct experimental or theoretical evidence for this. One suggestive result is that it is possible to denature (unfold) many proteins in... 

DNA BASE SEQUENCE = RNA BASE SEQUENCE = PROTEIN AMINO-ACID SEQUENCE The biopolymers provide striking evidence that the same atomic properties that give rise to covalent bonds, molecular shape, and intermolecular forces provide the means for all life forms to flourish. 

DNA analysis of the truncated Chi antenna size tlal insertional transformant Completed the DNA sequencing of the ARG7 insertion site in the tlal strain. Obtained full-length genomic DNA, cDNA and protein amino acid sequences for the TLAl gene. 

In fact, rarely more than 60 per cent of any protein amino-acid sequence is fitted to the databases, therefore peptide mass fingerprinting is remarkably efficient. Should peptide mass fingerprinting prove inadequate to identify a protein unambiguously, then tandem mass spectrometry may be required to further narrow the list of candidate proteins. 

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