Protein total internal reflection intrinsic

Proteins containing amino acids like tryptophan and tyrosine which intrinsically fluoresce upon excitation in the ultraviolet range can be employed, hence, the name total internal reflection intrinsic fluorescence-TIRIF, or... 

VanWagenen, R. A., Rockhold, S., Andrade, J. D., Probing Protein Adsorption Total Internal Reflection Intrinsic Fluorescence , in Interfacia Phenomena and Applications, Cooper, S. L., Pappas, N. A. (eds.) New York Wiley, 1982, p. 351. 

Total internal reflection intrinsic fluorescence (TIRIF) spectroscopy and molecular graphics have been applied to study the adsorption behavior of two lysozymes on a set of three model surfaces. A recently devised TIRIF quantitation scheme was used to determine adsorption isotherms of both hen egg-white lysozyme (HEWL) and human milk lysozyme on the three model surfaces. This preliminary study suggests that the adsorption properties of the two lysozymes are significantly different, and that further comparative studies of the two lysozymes might prove to be beneficial in understanding how protein structure might influence adsorption properties. Molecular graphics was used to rationalize the adsorption results from TIRIF in terms of the proteins surface hydrophobic/hydrophillic character. 

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