Protein receptor, abiotic

A year later the same research group presented a second rather different approach [160] in which they described the production of abiotic receptors by imprinting of proteins. The basic idea was once again to induce a certain conformation into the tertiary structure of a protein using a template and then to freeze it in . Bovine serum albumin (BSA) was dissolved in water, containing either p-hydroxybenzoic acid or tartaric acid. After lyophilisation the protein was washed with anhydrous solvent to remove the template. The resultant imprinted proteins bound up to 30-fold more of the template in anhydrous... 

A good example of using polymeric scaffold elements is imprinting proteins. Production of abiotic receptors was developed using bovine serum albumin (BSA) [55]. Kilvanov et al. utilized molecule aggregation of the protein during lyophiliza-tion in the presence of an L-malic acid template molecule (10.9). 

Braco, L. Dabulis, K. Klibanov, A.M. Production of abiotic receptors by molecular imprinting of proteins. Proc. Natl. Acad. Sci. USA 1990, 87, 274-277. 

On the basis of these promising results, the Mazik group systematically varied the substituents around the aromatic core to study their influence on carbohydrate binding. Substituents were chosen to mimic the functional groups involved in protein-carbohydrate interactions, and also effects of abiotic substituents such as crown ethers were studied. This work has recently been reviewed," and only receptors lOa-c are therefore additionally presented here because 10c is one of the few carbohydrate receptors for which a crystal structure with a bound guest has been obtained. 

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