Protein precipitation particle size distribution

Petanate, A.M. and Glatz, C.E., 1983. Isoelectric precipitation of soy protein. I. Factors affecting particle size distributions. II. Kinetics of protein aggregate growth and breakage. Biotechnology and Bioengineering, 25, 3049. 

Fisher, R. R. 1987. Protein precipitation with acids and polyelectrolytes The effects of reactor conditions and models of the particle size distribution. Ph.D. Thesis. Iowa State University, 165 pp. 

Figure 4.15 Particle size distribution of protein aggregate in a continuous stirred-tank reactor comparison of model with experimental data. 0.15kg/m A, 300kg/m O, 25.00kg/m. [From The Formation and Growth of Protein Precipitates in a Continuous Stirred-Tank Reactor, C.E. Glatz, M. Hoare, and J. Landa-Vertiz (1987), AIChE J. 32(7), pp. 1196-1204. Reproduced by permission of the American Institute of Chemical Engineers. 1987 AIChE.]...

Defatted oil seed meals are known to contain large amounts of nutritional proteins which can be recovered for use in food and pharmaceutical industries. Protein recovery from solution is often achieved through precipitation followed by solids-liquid separation. For efficient recovery, it is imperative to maximize the solids yield and the mean particle size, and minimize the spread of particle size distribution (PSD). In this research program, recovery of canola and sunflower protein precipitates has been investigated. Three different types of precipitators (batch, MSMPR, and tubular) and four different precipitants (HCl, carboxymethylcellulose (CMC), sodium... 

The PCA process is a slight modification of the ASES process. It is a one-step technique used to produce solvent free particles with a narrow size distribution at mild operating conditions [22]. It involves feeding SFCO into a precipitator so as to pressurize the precipitator to a desired value. The CO flow rate into the precipitator is then fixed. The solution of protein is fed into the precipitator through a nozzle. The antisolvent effect of the SECO leads to the precipitation of the protein particles. 

Figure 2. Particle (number) size distributions for isoelectrically precipitated soy protein showing the effects of shear rate and protein concentration. Points are experimental data curves are the model fit using Equation 12. Shear rates A, 417 s l ...

Precipitation Simple and convenient operation high yield Poor particle size control irregular-shaped particles broad size distribution protein denaturation difficult to handle multicomponents... 

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