Protein microassays

Protein concentration was determined by Bio-Rad protein microassay kit (Hercules, CA). 

PCE shell PEG core DMF/CHCI3 (3 1) water BSA, lysozyme Protein microassay Jiang et al. (2005)... 

Brumback [28] has described a custom robotic system designed to automate the extraction of proteins from plant samples. Leaf or callus material (5—25 mg) is presented to the robot in microcentrifuge tubes, the system performs buffer dispensing, grinding, centrifugation, and pipetting unit operations, and a cleared supernatant is delivered in a 96-well microassay plate format for subsequent analysis. 

The inflammatory reaction around eye bums has been subject of older research and might be more elucidated by means of modem technologies of microassays on molecular and proteinic levels. 

Wu, D., and Regnier, RE. Native protein separations and enzyme microassays by capillary zone and gel electrophoresis. Anal. Chem., 65, 2029-2035,1992. 

After purification the enzymes were precipitated by 80% ammonium sulphate in 250mM potassium phosphate, (containing O.SmM EDTA) pH 7.6 and stored at 4°C in an approximately 5-fold excess, of FAD. Prior to use, the enzyme was dissolved in 250mM KPi, 0.3 mM EDTA, pH 7.6, and run through a Sephadex G-25 column to remove the ammonium sulphate and excess FAD. The enzyme concentration was measured from the visible spectrum, taking 443 = 14,8001.mor. cm l Total protein was measured by the Bradford microassay and enzyme activity was measured by the ferricenium assay. ... 

Microassay For diluted samples (less than 0.1 mg ml ) proceed as follows Employ 200 1 of sample and add 50 fil of protein reagent stock. 

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