Prostanoids analysis

Samples for prostanoid analysis by GC-MS must be carefully prepared and purified high-performance liquid chromatography is particularly useful in this respect (Frolich, 1977). Mass spectrometric quantification is achieved through the use of stable isotopes as internal standards, e.g. 3,3,4,4fH 4-PGF2 (Fisher and Frolich, 1982). Gas-chromatographic separation is improved with suitable capillary columns (Rigaud eta/., 1981). 

Cyclic diethylsilylene and di-tert.-butylsilylene derivatives have been used to protect diol groups in steroids and prostanoids [539-541]. N,0-bis(diethylhydrogensilyl)trifluoro-methylacetamide simultaneously converts Isolated hydroxyl groups to the diethyllu ogensilyl ether allowing a single step derivatization toVp used for the analysis of corticosteriods. 

Narumiya, S., and EitzGerald, G. A. (2001) Genetic and pharmacological analysis of prostanoid receptor function. J. Clin. Invest. 108, 25-30. 

Investigating metabolism or stability of prostanoids, radiolabeled precursors or analytes are often used. The tritiated or -labeled compounds can easily be detected without any further derivatization using online or off-line liquid scintillation, which is not impaired by any interferences derived from matrix components. Efficient but less sensitive PG analysis is possible by UV detection (190-210 nm) of underivatized substances demanding the remove of interfering contaminants or simple sample matrices like buffers or some cell supernatants. 

Kapron, J. Wu, J. Mauriala, T. Clark, P. Purves, R.W. Bateman, K.P, Simultaneous analysis of prostanoids nsing liquid chromatography/high-field asymmetrie waveform ion mobility spectrometry/tandem mass spectrometry. Rapid Commun. Mass Spectrom. 2006, 20, 1504-1510. 

The presence of the TMS groups has little influence on fragmentation in the NICI mode [91,100,124], The hivourable GC-MS behaviour of the PFB—TMS and MO—PFB—TMS derivatives has made this the method of choice for the validation of other less specific assay techniques such as radioimmunoassay (RIA) [112, 117]. The GC—MS-MS behaviour of prostanoid ME—TMS, MO-ME-TMS [96-98, 1251 PFB-TMS and MO-PFB-TMS [116,125) derivatives has been investigated with a view to improving the selectivity of GC-MS analyses. Indications are that this approach is capable of achieving high sensitivities and selectivities in the analysis of prostanoids. 

Prostaglandins Isoprostanes and Synthetic Prostanoid Drugs Analysis by HPLC... 

Nowadays, especially pharmaceutical applications make use of the highly selective and saisitive LC-MS/MS methods for both qualitative and quantitative analysis. Depending on the structure of the synthetic prostanoids, product ions of highest inteisity goierated by CID may be derived from larger ntm-natural substituents, e.g., 3-tiifluoromethylphenolate (from travoprost or fluprostenol, Rg. 3). ... 

ANALYSIS OF PROSTANOIDS AND THEIR METABOLITES BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY... 

The objective of this contribution is to discuss still another method for the analysis of the prostanoids and similar compounds (the eicosanoids")/ used by other investigators to a limited extent compared with the three assays mentioned above, namely gas chromatography with electron-capture detection (GC-EC). 

---