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Yeast propagation

Studies with mammalian prion PrP have failed to identify any cellular factor, be it a protein, a co-factor or a nucleic acid species that is essential for the generation or propagation of PrPSc. This has led to the assertion that mammalian prions are self-replicating . Fungal prions are however different they require one or more cellular proteins in order to propagate successfully the prion form in vivo. This difference reflects in part the requirement to propagate yeast prions in a matter of... [Pg.278]

Moosavi B, Wongwigkam J, Tuite MF (2010) Hsp70/Hsp90 co-chaperones are required for efficient Hspl04-mediated elimination of the yeast PSf1 J prion but not for prion propagation. Yeast 27 167-179... [Pg.297]

The average viability (determined by methylene violet or methylene blue staining) of dried yeast is 20-30% lower than that of freshly propagated yeast. This problem can be accommodated by pitching according to viable cell numbers. [Pg.26]

Agar, which is low in metabolizable or inhibitory substances, debris, and thermoduric spores, is ideal for the propagation and pure culture of yeasts, molds, and bacteria. Agar also meets the other requirements of ready solubiUty, good gel firmness and clarity, and a gelation temperature of 35—40°C and a gel melting temperature of 75—85°C. A clarified and purified form of the bacterial polysaccharide, geUan gum, is the only known satisfactory substitute. [Pg.431]

Saccharomyces yeasts are rapid fermentors. S. cerevisiae and S. bayanus produce up to 18—20% ethanol. The cells are ovoid to spherical, eUiptical, or elongated (especially under conditions of nitrogen starvation). Vegetative propagation is by multilateral budding. S. uvarum and S. rosei occur earher in the fermentation, when S. rosei may produce up to 6—8% ethanol before being overgrown by the other Saccharomyces yeasts. S. cerevisiae may produce up to 18-20% ethanol (28). [Pg.392]

A Bacterial Artificial Chromosome (BAC) is a vector that allows the propagation of larger exogenous DNA fragments, up to several hundred kb. BACs are propagated in recombination-deficient strains of E. coli. They are more stable and easier to handle than yeast artificial chromosomes (YACs). [Pg.245]

An yeast artificial chromosome (YAC) is a vector that allows the propagation of large exogenous DNA fragments, up to several megabases, in yeast. [Pg.1482]

DePace, A. H., Santoso, A., Hillner, P., and Weissman, J. S. (1998). A critical role for amino-terminal glutamine/asparagine repeats in the formation and propagation of a yeast prion. Cell 93, 1241-1252. [Pg.174]

Paushkin, S. V., Kushnirov, V. V., Smirnov, V. N., and Ter-Avanesyan, M. D. (1996). Propagation of the yeast prion-like psi] determinant is mediated by oligomerization of the SUP35-encoded polypeptide chain release factor. EMBO J. 15, 3127-3134. [Pg.177]

Propagation constants, 20 219 Propagation plant, brewer s yeasts, 3 581 Propagation rate constants (kp), in VDC polymer degradation, 25 715-716, 717t Propagation reactions, 14 274... [Pg.764]

Technically, cDNAs are cloned into plasmids carrying an antibiotic selection marker for propagation in bacteria, a nutritional selection marker for selection of transfected yeast as well as the respective origins of replication. After amplification in bacteria, the plasmids are transfected into the corresponding auxothrophic yeast strain using similar methods as for bacteria and stably... [Pg.591]

The three best-known examples of biochemical oscillations were found during the decade 1965-1975 [40,41]. These include the peroxidase reaction, glycolytic oscillations in yeast and muscle, and the pulsatile release of cAMP signals in Dictyostelium amoebae (see Section V). Another decade passed before the development of Ca " " fluorescent probes led to the discovery of oscillations in intracellular Ca +. Oscillations in cytosolic Ca " " have since been found in a variety of cells where they can arise spontaneously, or after stimulation by hormones or neurotransmitters. Their period can range from seconds to minutes, depending on the cell type [56]. The oscillations are often accompanied by propagation of intracellular or intercellular Ca " " waves. The importance of Ca + oscillations and waves stems from the major role played by this ion in the control of many key cellular processes—for example, gene expression or neurotransmitter secretion. [Pg.261]

Yeast Artificial Chromosomes (YACs) YACs are used to clone large pieces of DNA in yeast cells. What three types of DNA sequences are required to ensure proper replication and propagation of a YAC in a yeast cell ... [Pg.947]


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See also in sourсe #XX -- [ Pg.16 ]

See also in sourсe #XX -- [ Pg.16 ]




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