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Principles of HPLC

In the following chapters, the basic principles of HPLC and MS, in as far as they relate to the LC-MS combination, will be discussed and seven of the most important types of interface which have been made available commercially will be considered. Particular attention will be paid to the electrospray and atmospheric-pressure chemical ionization interfaces as these are the ones most widely used today. The use of LC-MS for identification and quantitation will be described and appropriate applications will be discussed. [Pg.23]

This chapter presents an overview of current trends in high-pressure liquid chromatography (HPLC) instrumentation focusing on recent advances and features relevant to pharmaceutical analysis. Operating principles of HPLC modules (pump, detectors, autosampler) are discussed with future trends. [Pg.48]

HPLC precision is critical in pharmaceutical analysis.For most pharmaceutical assays under a good manufacturing practice (GMP) environment, retention time and peak area precision of <2.0% RSD must be demonstrated before any samples can be analyzed. This section reviews the fundamental principles of HPLC precision and offers practical guidelines for its enhancement. The reader is referred to Reference 18 for a more detailed treatment of this topic. [Pg.265]

Laboratory ergonomics Laboratory hoods Orientation to laboratory safety Planning for laboratory emergencies Principles of HPLC Principle and practice Principles of IR quantitative Qualitative and quantitative (slide show and discussion)... [Pg.984]

Application of some of the instrumentation principles of HPLC to electrophoresis, bearing in mind the need to cause all of the components of a mixture to migrate differently, has led to the development of several related techniques that are particularly useful in the amino-acid and peptide fields (Baker, 1995). A typical electro-pherogram (Figure 4.16) indicates the salient features of the capillary zone electrophoresis (CZE) analysis protocol. [Pg.83]

The following section aims to provide a brief description of the principles of HPLC and its most important parameters in water analysis. [Pg.168]

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See also in sourсe #XX -- [ Pg.258 ]



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