Preparative layer rotation planar chromatography

Rotation planar chromatography (RPC), as with OPLC, is another thin-layer technique with forced eluent flow, employing a centrifugal force of a revolving rotor to move the mobile phase and separate chemical compounds. The RPC equipment can vary in chamber size, operative mode (analytical or preparative), separation type (circular, anticircular, or linear), and detection mode (off-line or online). The described technique was applied in analytical and micropreparative separation of coumarin compounds from plant extracts. 

The main difference between the first four methods [normal chamber RPC (N-RPC), microchamber RPC (M-RPC), ultra-microchamber RPC (U-RPC), and column RPC (C-RPC) 1 lies in the size of the vapor space, that is an essential criterion in rotation planar chromatography. The circular mode of development is always used for on-line preparative separations by all of these methods. The sample is applied near the center of the layer, and the mobile phase is forced through the stationary phase from the center to the outside of the round plate/planar column. The separated compounds are eluted from the layer/planar column as a result of the centrifugal force and collected in a fraction collector. 

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