Preparative-layer chromatography

Methyl-1-phenylisoquinoline 2-oxide (l.OOg, 4.3 mmol) in acetone (200 mL) was irradiated with a Hanovia Q-700 medium-pressure Hg lamp until TLC showed that all starting material had been consumed. The solution was evaporated in vacuo, and the oily residue purified by preparative layer chromatography yield 0.485 g (48.5%) mp 73-75 C. 

PREPARATIVE LAYER CHROMATOGRAPHY AS A PRACTICAL USAGE OF THE NONLINEAR REGION... 

Modem planar chromatography is suitable not only for qualitative and quantitative analysis but also for preparative purposes. The separation efficiency of a thin-layer chromatographic system is independent of this intended purpose and is mainly determined by the quahty of the stationary phase, that is to say, by the applied coated layer. Therefore, progress in modem planar chromatography can be attributed not only to the development of the efficiency of the instmments but also to a large extent to the availability of high-quahty precoated layers. And today, as in the past, bulk sorbents for self production, especially of preparative layer chromatography (PLC) layers, are widely used. 

Planar chromatography has been used for preparative purposes in the past and is still used today. The reasons for the usage of preparative layer chromatography are, first of all, its cost-effectiveness and flexibility. 

From Nyiredy, Sz., Preparative layer chromatography, in Handbook of Thin-Layer Chromatography, 3rd ed., revised and expanded. Chromatographic Science Series, 89, Sherma, J. and Fried, B., Eds., Marcel Dekker, New York, 2003, chap. 11. 

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