Preparation cell surfaces

Electron micrographs of freeze-etched preparations clearly demonstrated that S-layers completely cover the cell surface during all stages of cell growth and division [5,56,57]. 

As part of a study on cell-surface glycans, 2,3-bis(tetradecyloxy)propyl 2-deoxy-2-fluoro-a- and - -D-mannopyranosides were prepared by condensation of 3,4,6-tri-0-benzyl-2-deoxy-2-fluoro-a-D-mannopyranosyl chloride with 2,3-bis(tetradecyloxy)propanol. 

Effect on the cell surface 4 Semi-solid antibacterial preparations... 

Most receptors (a notable exception being the steroid receptors that influence DNA transcription) are located on the cell surface, and purified cell membranes are thus an obvious choice of preparation. When a tissue is homogenized, however, any membrane fraction isolated may well contain membranes from intracellular organelles in addition to cell membranes from all the cell types present in the tissue. Thus, brain membranes will contain membranes not only from neurons but... 

Proteins can be immobilized on the cell surface with the use of a short, single stranded DNA (ssDNA) attached to the end of a PEG chain (ssDNA-PEG-lipid) [114—116, 119, 125]. First, an ssDNA-PEG-lipid is prepared by conjugating... 

Carbohydrates not only interact with protein receptors, but also with other carbohydrates and nucleic acids. Recently, gold nanoparticles (GNPs) have been prepared as new multivalent tools that mimic carbohydrate presentation on the cell surface. Using this tool, a weak calcium-mediated carbohydrate-carbohydrate interaction has been detected using NMR, employing... 

By preparing planar lipid monolayers or bilayers on hydrophobically derivatized or native hydrophilic glass, respectively, the adsorption equilibrium constants of a blood coagulation cascade protein, prothrombin, have been examined by TIRF on a surface that more closely models actual cell surfaces and is amenable to alterations of surface charge. It was found that membranes of phosphatidylcholine (PC) that contain some phosphatidyl-serine (PS) bind prothrombin more strongly than pure PC membranes/83... 

Lochner, N., Wirth, M., Pittner, F., Gabor, F., Preparation, characterization and application of artificial Caco-2 cell surfaces in the silver nanoparticle enhanced fluorescence technique. J Control Release 89, 249-259 (2003). 

Figure 2. Ultrastructural aspects of M813 virus-induced ceii fusion as shown by replica preparation technique, (a) Cell surface replica of a PA317 fibroblast after incubation for 30 min at 4°C with medium containing M813 virus. Numerous microvilli extend from the slightly folded cell surface. The...

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