Potassium channel shaker

Kv 3 -subunits are auxiliary subunits of Shaker-related Kv-channels, which belong to the Kvl subfamily of voltage-gated potassium channels. Kv(3 -subunits may function as chaperones in Kva -subunit assembly and may modulate the gating properties of Kv-channels. In particular, some Kv(3 -subunits may confer a rapid inactivation to otherwise non-inactivating Kv-channels. 

Shaker-channels, eag (ether- -go-go)-channels, slo (slow-poke)-channels were cloned from behavioral Drosophila melanogaster mutants. The channels were named according to the Drosophila mutant phenotype, Shaker, ether-go-go, slow-poke. Subsequently, eag-cDNA was used to clone related voltage-gated potassium channel subunits erg (eag-related) and elk (eag-like). The human erg ortholog (HERG) mediates cardiac DCS. 

Ding, S. et al. (2005) Investigating the putative glycine hinge in shaker potassium channel. J. Gen. Physiol. 126, 213-226. 

Hoshi, T., Zagotta, W. and Aldrich, R. W. Biophysical and molecular mechanisms of Shaker potassium channel inactivation. Science 250,533-538,1990. 

Panyi, G. and Deutsch, C. (2007) Probing the cavity of the slow inactivated conformation of Shaker potassium channels. The Journal of General Physiology, 129, 403-418. 

The Shaker potassium channel did not yield crystals for the first potassium ion channel X-ray crystallographic structure. Rather, it was the prokaryote bacterium Streptomyces lividans, abbreviated as the KcsA K+ channel, that first produced crystals suitable for crystallography. This structure, published in 1998 by MacKinnon s group in Science magazine, was received with great praise from the scientific community. It was known at that time that the amino acid sequence of KcsA was similar to that of other K channels, including vertebrate and invertebrate voltage-dependent K (Kv) channels (such as the... 

Shaker potassium channel), vertebrate inward rectifier channels (Ki,), Ca -... 

Galeotti, N., Ghelardini, C., Papucci, L., Capaccioli, S., Quattrone, Bartolini, A. An antisense oligonucleotide on the mouse Shaker-like potassium channel Kv1.1 gene prevents antinociception induced by morphine and baclofen, The Journal of Pharmacology and Experimental Therapeutics 1997a, 281, 941-949. 

Kim, E., Day, T.A., Bennett, J.L. and Pax, R.A. (1 995a) Cloning and functional expression of a Shaker-related voltage-gated potassium channel gene from Schistosoma mansoni (Trematoda Digenea). Parasitology 110, 1 71-180. 

Asamoah, O. K., Bezanilla, F., and Loew, L. (2002). Probing the electric field in the Shaker potassium channel. Biophys. J. 82, 233a. 

Glauner, K. S., Mannuzzu, L. M., Gandhi, C. S., and Isacoff, E. Y (1999). Spectroscopic mapping of voltage sensor movement in the Shaker potassium channel. Nature 402, 813-817. 

Schoppa, N. E., McCormack, K., Tanouye, M. A., and Sigworth, F. J. (1992). The size of gating charge in wild-type and mutant Shaker potassium channels. Science 255, 1712-1715. 

Sigg, D., Stefani, E., and Bezanilla, F. (1994). Gating current noise produced by elementary transition in Shaker potassium channels. Science 264, 578-582. 

The first voltage-gated potassium channel to be identified was the gene encoding the Shaker mutation in the fruit fly Drosophila. Figure 9.3 presents the hrst pictures of the tetrameric Shaker K+ channel with... 

Zagotta WN, Hoshi T, Aldrich W 1994 Shaker potassium channel gating. Ill evaluation of kinetic models for activation. J Gen Physiol 103 321—362... 

To demonstrate that we could immobilize a voltage sensor, we first turned to Shaker potassium channels, because (i) gating currents, which primarily reflect S4 movement, are easy to measure, and (ii) the channels have tetrameric symmetry. We used a non inactivating variant of Shaker into which we introduced the cysteine mutant A359C, located at the extracellular end of the S4 segment. After exposure to BPMTS each channel has four labelled S4 segments. 

Islas LD, Sigworth F J 2001 Electrostatics and the gating pore of Shaker potassium channels. J Gen Physiol 117 69—89... 

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