Polysorbate 80, quantitation

The opportunity of application of filters, solid sorbents and absorbing solutions has been investigated for synthetic pyrethroids concentrating from air. Silicagel KSK and polysorb are provided quantitative sorbption and desorbption pyrethroids. In case of aerosol formulations application of paper filters is possible. 

A multiwavelength approach might have been considered as an alternative to chemical derivatisation. Ruddle and Wilson [62] reported UV characterisation of PE extracts of three antioxidants (Topanol OC, Ionox 330 and Binox M), all with identical UV spectra and 7max = 277 nm, after reaction with nickel peroxide in alkaline ethanolic solutions, to induce marked differentiation in different solvents and allow positive identification. Nonionic surfactants of the type R0(CH2CH20) H were determined by UV spectrophotometry after derivatisation with tetrabromophenolphthalein ethyl ester potassium salt [34]. Magill and Becker [63] have described a rapid and sensitive spectrophotometric method to quantitate the peroxides present in the surfactants sorbitan monooleate and monostearate. The method, which relies on the peroxide conversion of iodide to iodine, works also for Polysorbate 60 and other surfactants and is more accurate than a titrimetric assay. 

Fatty acids can be determined easily by GC and HPLC. Hydrolysis of polysorbate 80 will release oleic acid. Thus, if a suitable hydrolysis procedure can be established to release oleic acid quantitatively, either a GC or an HPLC technique can be performed to determine the amount of polysorbate 80 in Nasonex. Approximately 9 g of Nasonex and an equivalent amount of polysorbate 80 standard were transferred into two separate flasks, followed by the addition of... 

Patty acids can be determined easily by GC and HPLC. Hydrolysis of polysorbate 80 will release oleic acid. Thus, if a suitable hydrolysis procedure can be established to release oleic acid quantitatively, eitlier a GC or an HPLC technique can be performed to determine tlie amount of polysorbate 80 in Nasonex. Approximately 9 g of Nasonex and an equivalent amount of polysorbate 80 standard were transferred into two separate flasks, followed by the addition of 1.0 mL of 1 N NaOH, and mixed well. The mixture was heated in a water batli at 70°C for 3 h. The sample and standard solutions were removed from tlie water batli and cooled to room temperature. An equal volume of acetonitrile was added to tlie solutions, mixed well, and centrifuged. The supernatant portion is filtered tlirough a 0.2-ttm nylon syringe filter. The hydrolysis can also be carried out under acidic conditions. Most of the procedures in tlie acidic conditions were similar to basic hydrolysis. The only difference was adjustment of tlie filial H2SO4 concentration to 1 A and extending the hydrolysis tune to 24 h. 

Other aspects of formulation such as the nature of the binary or ternary vehicle (oil-surfactant, water-surfactant or oil-water-surfactant, respectively) have been considered recently [151], Addition of polysorbate 80 to the aqueous phase has no significant effect on the epidermal transport of ethanol, but a significant reduction in the transport of the less soluble octanol results, in line with the arguments presented above in isopropyl myristate, octanol transport is not affected by the solubilizer while that of ethanol is decreased. In the ternary systems identified in Fig. 7.35, the results in Table 7.14 were obtained indicating a general decrease in permeability constants for ethanol, butanol and octanol. The viscosity of the vehicles was not a factor although this varied from 1 to 39 X 10 cP. In the ternary systems a surfactant will distribute itself between the aqueous and non-aqueous phase quantitative prediction of permeation is made difficult even with data on the transport properties of the permeants in the individual phase. The results indicate that the percutaneous absorption of the... 

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