Polymerase chain reaction principle

Introduction Immuno-Polymerase Chain Reaction—Principle of the Method. . 239... 

Fig. 8.2.2 Principle of genomic quantification by multiplex ligation-dependent probe amplification (MLPA). Synthetic oligonuleotides are designed to bind to exon 1 (oligo 1a and 1b) and 2 (2a and 2b). After specific hybridisation, the oligos are ligated. The ligation products undergo quantitative polymerase chain reaction using universal primers X and Y...

Figure 24-1 The basic principle underlying the technique of polymerase chain reaction (PCR).

The polymerase chain reaction (PCR) can clone (or amplify) DNA samples as small as a single molecule. If a length of DNA is mixed with the four nucleotides (A, T, C and G), and the enzyme DNA polymerase, then the DNA will be replicated many times. The principle of PCR is as follows ... 

Figure 5-16. Principle of the polymerase chain reaction (PCR). The polymerase chain reaction relies on primer-dependent replication of a double stranded DNA template. At the beginning of the reaction, the double stranded DNA is denatured and two primers complementary to defined regions on the two strands are hybridised to the separated strands by annealing.

Clavel C, Binninger I, Polette M, et al. [Polymerase chain reaction (PCR) and pathology. Technical principles and application]. Ann Pathol. 1993 13 88-96. 

IMMUNO-POLYMERASE CHAIN REACTION 13.4.1 History and Principle of IPCR Assays... 

The principle of in vitro selection is governed by a number of the same principles that apply to the Darwinian theory of evolution, as shown in Figure 2. First, the random sequence DNA is prepared by automated solid-phase synthesis. A mixture of four types of nucleotide is added in a stepwise condensation reaction process. When necessary, this DNA library may be converted to an RNA library by in vitro transcription or to a peptide library by in vitro translation. Second, the prepared DNA, RNA, or peptide library is subjected to affinity selection, and the molecules that bind to a target molecule are selected. Because only a very small part of the library is selected in each selection, the selected fraction is then amplified by a polymerase chain reaction (PCR) or a reverse transcription PCR (RT-PCR) technique. Successive selection and amplification cycles bring about an exponential increase in the abundance of the targeting DNA, RNA, or peptide until it dominates the population. 

---