Polymerase chain reaction buffers

List of Abbreviations PCR, polymerase chain reaction RT-PCR, reverse transcription polymerase chain reaction DNA, deoxyribonucleic acid RNA, ribonucleic acid RNase, ribonuclease mRNA, messenger RNA GABAa, y-aminobutyric acid type A cRNA, copy RNA dNTPs, deoxy nucleoside triphosphates MMLV, Mouse Moloney murine leukemia vims RT, reverse transcriptase bp, base pair Tm, melting temperature DEPC, diethylpyrocarbonate OD, optical density mL, milliliter SA-PMPs, streptavidin paramagnetic particles dT, deoxy thymidine DTT, dithiothreitol DNase, deoxyribonuclease RNasin, ribonuclease inhibitor UV, ultraviolet TBE, Tris-borate, 1 mM EDTA EDTA, ethylenediaminetetraacetic acid Buffer RET, guanidium thiocyanate lysis buffer PBS, phosphate buffered saline NT2, Ntera 2 neural progenitor cells... 

Polymerase chain reaction (PCR) Master Mix containing Taq DNA polymerase, deoxynncleotide 5 -triphosphate (dNTP), MgCl, and reaction buffers (Promega). 

First, a PCR is performed as described in 8.2.3.2 Polymerase Chain Reaction. The product is then digested by mixing 1.5 ploflOx enzyme reaction buffer, 8.5 pi water, 1 - 5 U enzyme and 5 pi PCR product. 

CFCF, continuous-flow cell-free protein synthesis system PCR, polymerase chain reaction TB, transcription buffer. 

The polymerase chain reaction uses (1) a thermostable DNA polymerase, such as Taq polymerase derived from the bacterial thermophile Thermus aquaticus, (2) a DNA template which is to be amplified, (3) two primers, each typically of around 20 nucleotides, which anneal to distinct parts on the complementary strands of the target and serve as sites for commencing DNA polymerase action, (4) a solution including the four deoxynucleoside triphosphates dATP, dCTP, dGTP and dTTP, Mg2+, salts and pH buffer. 

Polymerase Chain Reaction (PCR) Aptamer A9 was PCR amplified using lOx PCR buffer (100 mM Tris-HCl, 500 mM KC1, 15mM MgCl2, 0.1% Gelatine, pH 8.3), 4mM dNTP stock, 20 pM primer stocks, and Taq DNA polymerase (20 U, Invitrogen, Carlsbad, CA). 

DNA was extracted using an adapted protocol of Rogers and Bendich (1994). Double-stranded DNA templates were prepared by polymerase chain reaction (PCR). PCR was performed using a reaction mix of 47 il (1 il Taq polymerase, 5 il buffer, 3 il MgCl, 5 j,l dNTPs, 2 pi of each primer, 30 pi distilled water). Betaine was added to the reaction mix for unsuccessful ampMcations of ITS (12 pi betaine 5M for 50 pi of reaction mix). 

---