Poly uridylic acid

An attempt has been made to apply the Cohen and Reiss theory to dimer and hydrate formation in RNA.158 The results were inconclusive, probably because of a poor choice of example. Application of the theory to RNA was complicated by the necessity of estimating the distribution of uracil residues on the chain. The results are made still more tentative by the fact that Tanaka ignored the probability of dimer formation between cytosine residues, mixed dimers between cytosine and uracil, and hydrate formation in cytosine as well as the resultant deamination phenomena. A better choice of example would have been poly-uridylic acid. 

Vives, M., Gargallo, R., and Tauler, R., Study of the intercalation equilibrium between the polynucleotide poly(adenylic)-poly(uridylic) acid and the ethidium bromide dye by means of multivariate curve resolution and the multivariate extension of the continuous variation and mole ratio methods, Anal. Chem., 71, 4328 1-337, 1999. 

Some research over a number of years has been devoted to the existence of triple helix DNA, which results from the interaction of native and single stranded DNA [9-11]. The triple helix complex formed between two strands of poly(uridylic acid) and one strand of poly(adenylic acid) was first described in 1957 by Felsenfeld [4]. Later in 1959, Hoogsteen [12]... 

Poly(U) = poly(uridylic acid), poly(A) = poly(adenylic acid), poly(C) = poly(cytidylic acid), poly(G) = poly(guanylic acid), poly(I) = poly(inosinic acid), poly(iA) = poly(isoadenylic acid), poly(VA) - poly(9-vinyladenine), poly(VC) = poly(l-vinylcytosine), poly(VU) = poly(9-vinyluracil), poly(VHX) = poly(9-vinylhypoxanthine)... 

Poly-VAd can form polymer complexes with poly-VUr8 or with poly(uridylic acid)-(poly U)8 16. The formation of triple-stranded (2 poly U poly-VAd) and double-stranded (poly U poly-VAd) structures containing poly-VAd loops were proposed for the poly U poly-VAd system16. The formation of these complexes seems to depend on the reaction condition. 

Until recently 1 3C nmr studies of nucleic acids have not received the same attention as those of nucleosides and nucleotides. Poly-uridylic acid (poly U), polyadenylic acid (Poly ), and unfractionated baker s yeast transfer ribonucleic acid (tRNA) have been investigated by 13C nmr techniques (Table 11 Mantsch and Smith, 1972 Komoroski and Allerhand, 1972, 1974). Assignments for uridine monophosphates were based on changes in phosphorus-carbon splittings and on changes in the chemical shift of approximately 2-5 ppm on the phosphorylated carbon, which was caused by the deshielding effect of the phosphate group (Mantsch and Smith,... 

Poly(Phe) polyCphenylalanine) poly(U) poly(uridylic acid)... 

Scheme 8.14 Intra-chain hydrogen abstraction from the sugar moiety in poly(uridylic acid) involving an uracil radical formed by addition of an OH radical.

Although mechanistic details, which are discussed in the relevant literature [14, 59, 60], cannot be dealt with here, the following aspect should at least be pointed out an attack at the nucleobase might induce chemical alterations in the sugar moiety that eventually result in strand breakage. This applies, for example, to the intramolecular hydrogen abstraction suggested in the case of poly(uridylic acid) (see Scheme 8.14) [59]. 

A poly(uridylic acid)-cellulose resin has been prepared by coupling poly-(uridylic acid) oxidized with periodate at one end to a hydrazide derivative of carboxymethylcellulose. The resin was used as a column-bound ribosome system in studies of translocation in ribosomes. 

Poly(uridylic acid) is firmly adsorbed by cellulose, furnishing a derivative that was used in the purification of eukaryotic mRNAs. ... 

Purification of anti-(picryl T) suppressor factor Affinity chromatography of poly(uridylic acid) sequences and negative strands of Sindbis virus-specific RNA s... 

A (Scheme 1) was also reported to form complexes with poly(uridylic acid) [poly(U)j. Due to the shorter distance between adenines in 2A compared with that of poly(U), the complexes were proposed to be 2 poly(U)-2A triple-stranded, poly(U)-2A double-stranded, and poly(U)-25% adenine of 2A double-stranded [46]. 

The RNA analogue, 23A, formed a triplex with natural poly(uridylic acid) with the mole ratio of 1 2. While the natural triplex poly(riboadenylic acid) [poly(rA)]-poly(ribouridylic acid) [poly(rU)]-poly(riboadenylic acid) [poly(-rA)] is transformed via a duplex [poly(rA)-poly(rU)-l-poly(rA)] (T 62 °C) to single strands (T 44 °C ) [14], the complex 23A-poly(rU)-23A was melted directly to the individual strands [23A-I-23 A-fpoly(rU)] at T of 80 °C. Com-... 

Poly(adenylic acid) Poly(uridylic acid) Pteridine (reduced) Pyran copolymer... 

Water soluble nucleic acid analogs were prepared by grafting uracil, thymine, 5-fluorouracil, hypoxanthine, cytosine, and adenine derivatives containing a hydroxy group onto linear poly(ethyleneimine). These polymers contained about 90 unit% of the nucleic acid bases, and were easily soluble in water at neutral pH resign. The analogs were found to form complementary polymer complexes with each other and with polynucleotides. The interaction study was made on these polymers, and with polynucleotides poly(uridylic acid) poly(U), poly(adenylic acid) poly(A), poly(inosinic acid) poly(I) and poly(cytidylic acid) poly(C) in water. They were found to form polymer complexes with polynucleotide by specific base-base interaction in aqueous solution. 

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