Poly gene delivery

Lin C, Blaauboer CJ, Timoneda MM, Lok MC, van Steenbergen M, Hennink WE, Zhong Z, Feijen J, Engbersen JF (2008) Bioreducible poly(amido amine)s with oligoamine side chains synthesis, characterization, and structural effects on gene delivery. J Control Release 126 166-174... 

Zugates GT, Peng W, Zumbuehl A, Jhunjhunwala S, Huang YH, Langer R, Sawicki JA, Anderson DG (2007) Rapid optimization of gene delivery by parallel end-modification of poly(beta-amino ester)s. Mol Ther 15 1306-1312... 

Panyam J, Zhou WZ, Prabha S et al (2002) Rapid endo-lysosomal escape of poly(dl-lactide-co-glycolide) nanoparticles implications for drug and gene delivery. FASEB J 16 1217-1226... 

Kim H, Akagi T, Akashi M (2010) Preparation of CpG ODN-encapsulated anionic poly (amino acid) nanoparticles for gene delivery. Chem Lett 39 278-279... 

Formation of a complex between DNA and polycationic compounds appears to be the initial and quite possibly a critical parameter for nonviral gene delivery. Several synthetic vector systems, which are generally cationic in nature, including poly(lysines), cationic liposomes or various types of block copolymers and recently dendrimers, have been shown to self-assemble with plasmid DNA [13-15] [16]. Specific physicochemical properties manifested by these DNA complexes depend on the type of cationic agent used however, interesting patterns for such interactions are beginning to evolve [17, 18]. Under certain conditions, the interaction of DNA with polyvalent cations results in... 

Li WL, et al. Low-pH-sensitive poly(ethylene glycol) (PEG)-stabilized plasmid nanolipoparticles effects of PEG chain length, lipid composition and assembly conditions on gene delivery. J Gene Med 2005 7 67. 

Akinc A, Anderson Daniel G, Lynn David M, Danger R (2003) Synthesis of poly(j3-amino ester)s optimized for highly effective gene delivery. Bioconjug Chem 14 979-988... 

Godbey WT, Wu KK, Mikos AG (1999) Poly(ethylenimine) and its role in gene delivery. J Controlled Release 60 149-160... 

Poly (ethylenimine) (PEI) has been demonstrated as an efficient gene delivery vehicle both in vitro and in vivo (161-163). Linear (22 kDa) and branched PEI formulations of varying molecular weights (0.6-800 kDa) have been reported. While polyplexes from higher molecular weight branched PEIs (70-800 kDa) were found to be more efficient in vitro but on intravenous administration the smaller and linear PEIs seem in general to be more efficient (171). However, questions as to the... 

Poly-(3-hydroxybutanoic acid) (PHB), belongs to the large family of poly-(hydroxyalkanoates) (PHAs), high molecular weight natural polymers produced by various microorganisms and stored in cell cytoplasm (200). Low molecular weight PHB, also present in bacteria and are primarily involved in transport of ions and DNA across inner bacterial membrane (201). PHB could be developed as a valuable biocompatible material with possible applications in gene delivery after cytotoxic, safety, and efficacy evaluations. 

FIGURE 13.6 Viral-nonviral hybrid vectors. DNA is bound to a poly-L-lysine (PLL)-trans-ferrin conjugate (A) to form a PLL-transferrin-DNA complex (B). Transferrin binds to specific receptors on the surface of some cancer cells, thereby targeting gene delivery to these cells (C). 

Read, M.L., Etrych, T., Ulbrich, K., Seymour, L.W. (1999). Characterisation of the binding interaction between poly(L-lysine) and DNA using the fluorescamine assay in the preparation of non-viral gene delivery vectors. FEBS Lett., 461(1-2), 96-100. 

Cherng, J.Y., Talsma, H., Crommelin, D.J.A., Hennink, W.E., (1999). Long term stability of poly((2-dimethylAm.ino)ethyl methacrylate)-based gene delivery systems. Pharm. Res., 16, 1417-1423. 

Godbey, W.T., Wu, K.K. and Mikos, A.G. (1999) Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery. Proc. Natl. Acad. Sci. USA, 96, 5177-5181. 

Table 9.1 Selected poly cations used for preparation of polyplexes for gene delivery...

Banaszczyk, M.G., Lollo, C.P., Kwoh, D.Y., Phillips, AT, Amini, A, Wu,O.P., Mullen, P.M., Coffin, C.C., Brostoff, S.W. and Carlo, D.J. (1999) Poly-L-lysine-graft-PEG comb-type polycation copolymers for gene delivery. J. Macromol. Sci., Pure Appl. Chem., A36, 1061-1084. 

Benns, J.M., Choi, J.S., Mahato, R.I., Park, J.S. and Kim, S.W. (2000) pH-sensitive cationic polymer gene delivery vehicle V-Ac-poly(l- histidine)-graft-poly(l- lysine) comb shaped polymer. Bioconjug. Chem., 11, 637-645. 

Toncheva, V., Wolfert, M.A., Dash, P.R., Oupicky, D., Ulbrich, K., Seymour, L.W. and Schacht, E.H. (1998) Novel vectors for gene delivery formed by self-assembly of DNA with poly(L-lysine) grafted with hydrophilic polymers. Biochim. Biophys. Acta, 1380, 354-368. 

Byk, G., Soto, J., Mattler, C., Frederic, M. and Scherman, D. (1998b) Novel non-viral vectors for gene delivery Synthesis of a second generation library of mono-functionalized poly-(guanidinium)amines. Biotech. Bioeng., 61, 81-87. 

POLY(L-LYSINE)-BASED GENE DELIVERY SYSTEMS... 

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