Gene delivery polymers

AV Kabanov, FC Szoka Jr, LW Seymour. In PF AV Kabanov, LW Seymour, eds. Interpoly-electrolyte Complexes for Gene Delivery Polymer Aspects of Transfection Activity. Chichester, UK Wiley, 1998, pp 197-218. 

Kabanov, A.V., Szoka, F.C. and Seymour, L.W. (1998b) Interpolyelectrolyte complexes for gene delivery polymer aspects of transfection activity. In A. V.Kabanov, P.L.Felgner and L.W.Seymour (eds) Self-assembling Complexes for Gene Delivery. From Laboratory to Clinical Trial. Chichester, UK John Wiley Sons. pp. 197-218. 

Gene delivery polymer(s). From synthesis in Subheading 2.1. and/or bought commercially such as PEI, A/w 25 kDa branched from Sigma or PBAEs can be bought directly from Open Biosystems as Leopard Transfection Array polymers. 

Fig. 1. Polymerization of 1,4-butanediol diacrylate and 5-amino-l-pentanol to form gene delivery polymer C32.

Lynn, D. M. Anderson, D. G. Akinc, A. Longer, R. Degradable poly(P-amino ester)s for gene delivery. Polym. Gene Dehv. 2005, 227-241. 

Georgiou TK (2014) Star polymers for gene delivery. Polym Int 63 1130-1133... 

By recording a series of images over the same region of a sample, it is possible to monitor biological processes as a function of time and build up animations of realtime processes. This approach has, for example, enabled the study of processes which include protein fibrillization, the transcription of DNA by RNA polymerase, the translocation of molecules through membrane pores/channels and the enzymatic degradation of potential gene-delivery polymer-DNA complexes. 

Fig. 1. Structures of several off-the-shelf gene delivery polymers.

Many of the early studies on polymer-mediated gene delivery employed commercially available poljuners. As they were not designed for gene delivery, their efficacy as delivery vectors is somewhat serendipitous. Off-the-shelf poisoners have nonetheless been very widely studied and form the basis for much of the gene delivery polymer literature. However, significant problems face these polymers, most... 

Dubmel P, Strycker JD, Westbroek P, Bracke K, Temmcaman E, Vandtavoort J, Ludwig A, Schacht E (2002) Synthetic polyamines as vectius for gene delivery. Polym Int 51 948-957... 

Teasdale, I., Briiggemann, O., 2013. Polyphosphazenes multifunctional, biodegradable vehicles for drug and gene delivery. Polymers 5, 161—187. 

Gebhart, C. L., S. Sriadibhatla, S. Vinogradov and A. Kabanov, 2001, Pluronic-polyethyleneimine conjugates for gene delivery. Polym. Prepr. (Am. Chem. Soc., Div. Polym. Chem.) 42 119-120. 

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